افزایش بیان ژن چاویکول o-متیل ترانسفراز و فعالیت آنزیمهای کاتالاز و آسکوربات‌پراکسیداز گیاه Ocimum basilicum L. تحت تأثیر کیتوزان

نوع مقاله: علمی پژوهشی

نویسندگان

1 دانشجوی دکتری اصلاح‌نباتات، دانشگاه زابل

2 دانشیار، گروه اصلاح نباتات و بیوتکنولوژی ،دانشکده کشاورزی، دانشگاه زابل

3 استادیار، گروه زیست‌شناسی، دانشکده علوم پایه، دانشگاه زابل

چکیده

ریحان (Ocimum basilicum L.) گیاه دارویی از خانواده نعناعیان (Lamiaceae) است که در طب سنتی ایران مورد استفاده قرار میگیرد. اسانس ریحان سرشار از ترکیبات فنیل پروپانوئیدی است. متیل چاویکول و متیل اوژنول از مهم‌ترین اجزا سازنده اسانس ریحان به شمار می‌روند. کیتوزان از ترکیبات اصلی دیواره سلولی بسیاری از گونه‌های قارچی است که به عنوان الیسیتور زیستی برای بهبود بخشیدن بیوسنتز متابولیت‌های ثانویه استفاده می‌شود. در این پژوهش، اثر کیتوزان بر میزان متیل چاویکول و بیان ژن چاویکول o-متیل ترانسفراز (CVOMT) و فعالیت آنزیم‌های آنتی اکسیدان کاتالاز و آسکوربات پراکسیداز مورد بررسی قرار گرفت. بدین منظور گیاهچههای ریحان در مرحله پیش‌گلدهی با غلظت 2 گرم در لیتر کیتوزان تیمار شدند و سپس در زمان‌های 1، 2، 3 و 5 روز بعد از اعمال تیمار کیتوزان برداشت شدند. آنالیز اسانس نشان داد که میزان متیل‌چاویکول تحت تأثیر کیتوزان نسبت به نمونه‌های شاهد افزایش یافت. همچنین بیان ژن چاویکول o-متیل ترانسفراز و فعالیت آنزیم‌های آنتی‌اکسیدان کاتالاز و آسکوربات پراکسیداز تحت تأثیر کیتوزان به طور معنیداری افزایش یافت. تغییرات میزان بیان ژن با روند تغییرات متیل چاویکول در مراحل مختلف برداشت مطابقت داشت. بنابراین کیتوزان از طریق افزایش بیان ژن چاویکول o-متیل ترانسفراز باعث افزایش متیل‌چاویکول گردید.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Increasing of Chavicol o-Methyl Transfrase Gene Expression and Catalase and Ascorbate Peroxidase Enzymes ctivity of Ocimum basilicum by Chitosan

نویسندگان [English]

  • Saleh Naderi 1
  • Baratali Fakheri 2
  • Sedigheh Esmailzadeh Bahabadi 3
1 Ph.D student of Plant Breeding, Zabol University, Zabol, Iran.
2 Associate Professor, Department of Plant Breeding and Biotechnology, Zabol University, Zabol, Iran.
3 Assistant Professor, Department of Biology, Zabol University, Zabol, Iran.
چکیده [English]

Basil (Ocimum basilicum L.), a medicinal plant of the Lamiaceae family, is used in traditional Iranian medicine. Essential oils of basil are composed of phenylpropanoids. Chavicol o-methyl and eugenol o-methyl are the most important components in essential oil of basil. Chitosan, main compound of fungal species could be used as biotic elicitor to improve secondary metabolites. In the present study, the effect of chitosan on methylchavicol content, chavicol o-methyl transfrase gene expression and enzymes activity of catalase, ascorbate peroxidase was evaluated. The plants were treated at pre flowering stage with 2 g/L chitosan and harvested after 1, 2, 3, and 5 days. Essential oils analysis showed that methylchavicol increased under chitosan compare to untreated plants. Chavicol o-methyl transfrose gene expression and antioxidant enzymes activity increased significantly after chitosan. Totally, changes in gene expression in different harvest stages are consistent with methylchavicol changes. Thus, chitosan increased methylchavicol by increasing CVOMT gene expression.

کلیدواژه‌ها [English]

  • : essential oils
  • Gene expression
  • Ocimum basilicum
  • CVOMT
  • antioxidant enzyme activity
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