مطالعه تغییرات رونوشت ژن های فنیل آلانین آمونیا-لیاز2 (PAL2) و پروتئین شبه توماتین (TLP) در ژنوتیپ های آفتابگردان در پاسخ به جدایه های قارچ عامل بیماری ساقه سیاه

نوع مقاله: علمی پژوهشی

نویسندگان

1 دانشجوی کارشناسی‌ارشد گروه اصلاح و بیوتکنولوژی گیاهی، دانشکده کشاورزی، دانشگاه ارومیه

2 استادیار گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه مراغه

3 دانشیار گروه اصلاح و بیوتکنولوژی گیاهی، دانشکده کشاورزی، دانشگاه ارومیه

چکیده

بیماری ساقه سیاه یکی از مهم‌ترین بیماری‌های قارچی آفتابگردان است. استفاده از ژنوتیپ‌های مقاوم به این بیماری یکی از اقتصادی‌ترین روش‌های کنترل آن است. در این مطالعه، میزان رونوشت ژن‌های فنیل آلانین آمونیا- لیاز 2 (PAL2) و پروتئین شبه توماتین (TLP) در ژنوتیپ‌های AS613 و ENSAT-B5 و ژنوتیپ جهش یافته M5-54-1 آفتابگردان بعد از آلودگی با جدایه‌های MA6، MP8 و MP10 قارچ Phoma macdonaldii Boerema عامل بیماری ساقه سیاه آفتابگردان با روش RT-PCR کمی بررسی شد. نتایج نشان داد که سطح رونوشت ژن‌های PAL2 و TLP به طور معنی‌داری تحت تاثیر جدایه، ژنوتیپ و اثر متقابل ژنوتیپ- جدایه می‌باشد. در این مطالعه، میزان رونوشت ژن های PAL2 و TLP در برابر جدایه MP8 بیشترین افزایش را نشان داد. در بین ژنوتیپ‌های مورد مطالعه، میزان رونوشت ژن‌های PAL2 و TLP در ژنوتیپ ENSAT-B5 بیشترین افزایش را نشان داد. ژنوتیپ‌های مقاوم و حساس به ترتیب دارای سطح بالا و پایین از رونوشت ژن های PAL2 و TLP بودند.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Study of changes in the phenylalanine ammonia-lyase 2 (PAL2) and thaumatin-like protein (TLP) genes transcripts in sunflower genotypes in response to fungi isolates of Black stem disease

نویسندگان [English]

  • Arezo Mohamadian Farsani 1
  • Hamid Hatami Maleki 2
  • Reza Darvishzadeh 3
  • Faramarz Hoshyardel 1
1 Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Urmia University, Urmia, Iran.
2 Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Maragheh, Maragheh, Iran
3 Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Urmia University, Urmia, Iran.
چکیده [English]

Black stem disease is one of the most important fungi diseases of sunflower. The use of resistant genotypes is potentially one of the economical ways for its control. In this study, expression level of genes including phenylalanine ammonia-lyase 2 (PAL2) and thaumatin-like protein (TLP) were measured in sunflower genotypes including ENSAT-B5, AS613 and mutant genotype M5-54-1 infected with MA6, MP8 and MP10 isolates of Phoma macdonaldii via quantitative RT-PCR technique. Results revealed that transcript levels of the both PAL2 and TLP genes were significantly affected by isolate and genotype and genotype-isolate interactions. In this study, the expression levels of genes PAL2 and TLP showed higher increase against the MP8 isolate. Among studied genotypes, genotype ENSAT-B5 possessed highest increasing in expression levels of genes PAL2 and TLP. The resistant and susceptible genotypes had possessed high and low transcript levels of PAL2 and TLP and disease symptoms were seen in susceptible genotypes.

کلیدواژه‌ها [English]

  • sunflower
  • Black stem disease
  • Phenylalanine ammonia-lyase2
  • Thaumatin like protein
  • Quantitative RT-PCR

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