عنوان مقاله [English]
Melon Vascular wilt caused by a soil-borne pathogen, Fusarium oxysporum f. sp melonis (Fom) is a distractive disease. The use of resistant cultivars is an effective way for controlling this fungus. Clear view about the interaction between virulence and resistant genes could provide applicable knowledge to design breeding programs. Fungus effector genes play a critical role during the plant-pathogen interactions. Exploring for the homologous of effector genes in different formae speciales started since some effector genes have been reported from Fusarium oxysporum f. sp. lycopersici (Fol). Recently, using bioinformatics assisted approach a homologous Fol-SIX1 effector gene has been reported from Fom. Here we performed a functional analysis study to reveal interactions between Fol-SIX1 and the melon differential varieties harboring defined R genes. Coding sequence of Fol-SIX1 from the pTS1 construct was subcloned in the pCAMBIA3301 binary vector in three steps and confirmed by colony PCR and digestion analysis. The accuracy of the expression construct, pCaS1 was evaluated by bidirectional sequencing using the PSh4-F2 and PSh51-R primers. Agroinjection-mediated transient expression approach was used to express Fol-SIX1 in leaves of the melon varieties. Functional analysis of Fol-SIX1 was performed by agroinjection of LBA4404 carrying pCaS1 into the leaves of melon lines. the leaves of Ch-T and Ch-Fom2 varieties developed a green dried symptom in response to transiently expressed Fol-SIX1 at 24 hours post injection (hpi), while Ch-Fom1 and BG lines did not responsive to the infection until 48 hpi.
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