بررسی حضور جایگاه‌های ژنی مقاومت به بیماری زنگ زرد و زنگ سیاه (ساقه) در لاین‌های دابل هاپلویید گندم نان با استفاده از نشانگرهای مولکولی

نوع مقاله: علمی پژوهشی

نویسندگان

1 دانشجوی دوره دکتری پردیس کشاورزی و منابع طبیعی دانشگاه رازی کرمانشاه، کرمانشاه

2 استاد گروه زراعت و اصلاح نباتات، پردیس کشاورزی و منابع طبیعی دانشگاه رازی کرمانشاه، کرمانشاه

3 استاد بخش تحقیقات غلات، موسسه تحقیقات اصلاح و تهیه نهال و بذر، کرج

4 دانشیار بخش تحقیقات غلات، موسسه تحقیقات اصلاح و تهیه نهال و بذر، کرج

چکیده

در این تحقیق با استفاده از روش حذف کروموزومی تلاقی گندم و ذرت 150 لاین دابل‌هاپلویید گندم تولید شد. لاین‌های تولید شده به همراه والدین و ارقام شاهد در دو مرحله گیاهچه و گیاه کامل نسبت به بیماری‌های زنگ زرد و زنگ سیاه گندم ارزیابی شدند و هشت جایگاه ژنی مقاومت به بیماری شامل Yr5، Sr31/Yr9/Lr26، Yr15، Sr38/Yr17/Lr37، Lr34/Yr18/Pm38، Yr27، Yr36 و Yr48 در والدین جمعیت‌ها مورد بررسی قرار گرفت. نتایج این بررسی نشان داد که نشانگرهای مورد استفاده برای مکان‌های ژنی Yr5، Yr15،Yr27 و Yr36 قادر به تشخیص پلی‌مورفیسم بین والد‌ها، شاهد حساس بولانی و کنترل‎های مثبت نبودند. در مکان‌های ژنی Sr38/Yr17/Lr37 و Yr48 عدم تکثیر باندهای موردنظر بیان‌کننده عدم حضور آلل موثر این جایگاه‎های ژنی در والدین لاین‌های دابل‌هاپلویید بود. در این تحقیق بلوک ژنی Sr31/Yr9/Lr26 در ارقام MV17 و فلاندرز مشاهده شد. بررسی لاین‌های دابل‌هاپلویید نسبت به این بلوک ژنی نشان داد که تنها سه لاین از جمعیت DH-26:Ghods*3/MV17 دارای بلوک‎ژنی Sr31/Yr9/Lr26 بودند که دو لاین از آنها نسبت به نژادهای زنگ سیاه TTSTK و TTKSK واکنش مقاومت نشان دادند. آزمون ژنتیکی حضور و یا عدم حضور آلل موثر بلوک ژنی Lr34/Yr18/Pm38 بر روی والدین لاین‌های دابل‌هاپلویید بیان کننده حضور آلل موثر این بلوک ژنی در والد MV17 بود. بررسی لاین‌های دابل‌هاپلویید نسبت به این بلوک ژنی نشان داد که شش لاین دابل‌هاپلویید از جمعیت DH-26:Ghods*3/MV17 حاوی بلوک ژنی Lr34/Yr18/Pm38 بودند که تنها یک لاین از آنها نسبت به نژاد زنگ زرد 7E158A+,Yr27 دارای واکنش مقاومت در مراحل گیاهچه و گیاه کامل بود.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Study on the presence of yellow and stem rust resistance genes in doubled haploid lines of bread wheat using molecular markers

نویسندگان [English]

  • Farshad Bakhtiar 1
  • Ezatollah Farshadfar 2
  • Mostafa Aghaee Sarbarzeh 3
  • Habibollah Ghazvini 4
  • Farzad Afshari 3
1 Ph.D. Student, Department of Crop Production and Plant Breeding, Campus of Agriculture and Natural Resources, Razi University, Kermanshah, Iran
2 Professor, Department of Crop Production and Plant Breeding, Campus of Agriculture and Natural Resources, Razi University, Kermanshah, Iran.
3 Professor, Cereal Research Department, Seed and Plant Improvement Institute, Karaj, Iran
4 Associate Professor, Cereal Research Department, Seed and Plant Improvement Institute, Karaj, Iran.
چکیده [English]

In this research, 150 wheat doubled haploid lines were produced using chromosome elimination method by crossing between wheat and maize. Resistance of doubled haploid lines, their parents and check cultivars against strip and stem rust was evaluated at seedling and adult plant stages. Accordingly, eight known molecular markers which are tightly linked to resistance genes including Yr5, Sr31/Yr9/Lr26, Yr15, Sr38/Yr17/Lr37, Lr34/Yr18/Pm38, Yr27, Yr36 and Yr48 were screened in parents. Results showed that molecular markers for Yr5, Yr15, Yr27 and Yr36 couldn't detect polymorphism between parents as well as positive and negative controls. For gene block Sr38/Yr17/Lr37 and locus Yr48 allele sizes were not similar to those which were expected for these genes. Results also showed that MV17 and Flanders have gene block of Sr31/Yr9/Lr26, and only 3 lines of population DH-26: Ghods*3/MV17 had this gene block from which two doubled haploid lines showed resistance reaction to TTSTK and TTKSK of Puccinia graminis pers. f.sp tritici races. Genetic test for the presence or absence of gene block Lr34/Yr18/Pm38 on parents of doubled haploid lines showed that MV17 comprises this gene block. Evaluation of doubled haploid lines for this gene block showed that 6 doubled haploid lines of population DH-26:Ghods*3/MV17 have gene block Lr34/Yr18/Pm38 for which only one doubled haploid line showed resistance reaction to Puccinia striiformis Westend f.sp. tritici race of 7E158A+, Yr27 at both seedling and adult plant stages.

کلیدواژه‌ها [English]

  • Wheat
  • Rust disease
  • Molecular markers
  • Doubled haploid
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