بررسی بیان افتراقی پروتئین های محافظ در گیاه آرابیدوپسیس تحت تنش شوری با روش iTRAQ-2DLC-MS/MS و آنالیز شبکه ژنی مربوطه

نوع مقاله: علمی پژوهشی

نویسندگان

1 دانشجوی دکتری اصلاح نباتات، دانشگاه علوم کشاورزی و منابع طبیعی ساری

2 استادیار و عضو هیئت علمی دانشگاه علوم کشاورزی و منابع طبیعی ساری.

چکیده

شناسایی و کمیتسنجی پروتئینها و ژنهای بیان شده در شرایط تنش شوری میتواند منجر به درک هرچه بهتر مکانیسمهای پاسخی شود. بدین منظور در این تحقیق از روش پروتئومیکس مقایسهای iTRAQ جهت بررسی تغییرات پروتئینی در شرایط تنش شوری و کمیتسنجی آنها استفاده شد. سپس جهت بررسیهای بیشتر پیرامون عملکرد ژنهای شناسایی شده از اطلاعات بیان ژن و همبیانی در پایگاهداده و آنالیز توالی‌های مربوطه استفاده شد. با توجه به نتایج به‎دست آمده از iTRAQ-2DLC-MS/MS مجموعهای از پروتئینها از جمله P5CS1، KIN1، KIN2، ERD10، ERD14 و COR47 شناسایی شد. نتایج بیان ژنهای مربوطه نشان داد که بیان این ژنها تنها به تنش شوری محدود نمیشود و در پاسخ به سایر تنشهای اسمزی نیز دخالت دارند. شبکهی ژنی پروتئینهای شناسایی شده با نرمافزار String بررسی گردید. بر اساس نتایج به‎دست آمده در طی بیان این ژنها ترکیبات محافظ از جمله مواد محلول سازگار، پروتئین‌های دهایدرین و غیره در سلول تولید میشود که حضور آنها منجر به القای مکانیسمهای مقاومت و تحمل در گیاه در برابر تنشهای اسمزی از جمله شوری می شود.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Investigation of differential protein expression of protective proteins under salinity stress in Arabidopsis by iTRAQ-2DLC-MS/MS and analysis of gene expression and related gene network

نویسندگان [English]

  • Mohammad Amin Baghery 1
  • Hamid Najafi Zarini 2
1 Ph.D Student of Plant Breeding, Sari Agriculture Sciences and Natural Resources University (SANRU), Sari, Iran
2 Assistant Professor of Plant Biotechnology, Sari Agriculture Sciences and Natural Resources University (SANRU), Sari, Iran.
چکیده [English]

Identification and quantification of proteins and genes that expressed in the salinity stress conditions could lead to better understanding of the response mechanisms. So, in this study iTRAQ comparative proteomic method was used to investigate the protein changes and quantification of them under salinity stress. Then, more studies about the function of identified genes were done using gene expression and co-expression data obtained from databases and analysis of related sequences. According to the results of iTRAQ-2DLC-MS/MS a set of proteins including P5CS1, KIN1, KIN2, ERD10, ERD14 and COR47 were identified. Results showed that expressions of the related genes were not just restricted to salinity stress. They were also involved in other osmotic stresses. Gene network based on identified proteins was evaluated by String software. Based on the results, during the expression of these genes, protective compounds such as compatible solutes, dehydrin proteins and etc. were produced in the cells. Presence of the compounds led to induction of resistance and tolerance mechanisms in plants against osmotic stress such as salinity.

کلیدواژه‌ها [English]

  • Arabidopsis
  • iTRAQ Comparative proteomics
  • Protective compounds
  • Gene network
  • Salinity

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