بررسی بیوانفورماتیکی و بیان خانواده ژنی SnRK2 در گیاه جو

نوع مقاله: علمی پژوهشی

نویسندگان

1 کارشناسی‌ارشد، گروه زیست شناسی سیستم ها، پژوهشکده بیوتکنولوژی کشاورزی ایران، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران

2 استادیار گروه زیست شناسی سیستم ها، پژوهشکده بیوتکنولوژی کشاورزی ایران، سازمان تحقیقات، آموزش و ترویج کشاورزی کرج، ایران

چکیده

تنش‌های غیرزیستی از مهم‌ترین عوامل محدود ‎کننده‌ی عملکرد گیاهان زراعی و پروتئین کینازهای SnRK2 از تنظیم‎کننده‌های کلیدی پاسخ گیاهان به تنش‌های غیرزیستی می‌باشند. با توجه به اهمیت اقتصادی، سطح زیر کشت و تحمل گیاه جو به تنش‌های غیرزیستی، در این تحقیق اعضای خانواده‌ی SnRK2 در گیاه جو شناسایی و مورد بررسی قرار گرفته‌اند. به‎همین منظور توالی‌های حفظ شده خانواده SnRK2 در پایگاه‌های اطلاعاتی مختلف همچون NCBI و پروژه ژنوم جو، با ابزار tBLASTn در بین توالی‌های ثبت شده برای گیاه جو جستجو شدند. در نتیجه 10 عضو یافت شدند HvSnRK2.1) تا HvSnRK2.10) که 8 عضو آن تاکنون گزارش نشده بودند. اعضای خانواده‌ی SnRK2 جو با اعضای این خانواده در آرابیدوپسیس و برنج همردیف و درخت فیلوژنتیک رسم شد. تعیین جایگاه کروموزومی، آنالیز پروموتر و تعریف ساختار ژن‌ها انجام شد و الگوی بیان هر ژن در اندام‌ها، تیمارها و ارقام مختلف بر اساس داده‌های ریزآرایه مورد بررسی قرار گرفت. نیمی از اعضای این خانواده روی کروموزوم 2 و بقیه روی کروموزوم‌های 1، 4، 5 و 6 قرار داشتند. تعداد اینترون در اعضای این خانواده از صفر تا 8 متغیر بود. 19 نوع عامل سیس شامل عوامل مؤثر در پاسخ به تنش‌های غیرزیستی، بر روی پروموتر اعضای خانواده ژنی HvSnRK2 شناخته شد. بیان ژن HvSnRK2.6 توسط خشکی، شوری و سرما القا شده و به‎نظر می‌رسد در انتقال پیام این تنش‌ها و ایجاد تحمل به آنها نقش مهمی ایفا کند. امید است بتوان از این ژن برای اصلاح و دست‌ورزی گیاهان در جهت تحمل به تنش‌های غیرزیستی به‎ویژه خشکی بهره برد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

In silico characterization and expression analysis of SnRK2 family in barley

نویسندگان [English]

  • Yaser Panahi Fakoor 1
  • Zahra-Sadat Shobbar 2
  • Ehsan Pourabed 1
  • Farzan Ghane Golmohamadi 1
  • Seyed Morteza Razavi 1
1 M.Sc., Department of Systems Biology, Agricultural Biotechnology Research Institute of Iran, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
2 Assistant Professor, Department of Systems Biology, Agricultural Biotechnology Research Institute of Iran, Education and Extension Organization (AREEO), Karaj, Iran
چکیده [English]

Abiotic stresses are among major factors limiting crop yields, and SnRK2 protein kinases are one of the key regulators of plant response to abiotic stresses. Due to the economic importance, cultivation area, and tolerance of barley to the abiotic stresses, identification and characterization of SnRK2 family members in barley is performed in present research. SnRK2 conserved sequences were used as a query for tBLASTn analysis in different databases such as NCBI and international barley sequencing consortium against all of the reported barley sequences. As a result, 10 members were identified (HvSnRK2.1 to HvSnRK2.10) which 8 of them were not yet reported. These HvSnRK2 members were aligned with AtSnRK2s and OsSnRK2s and a phylogenetic tree was constructed. Detection of chromosomal localization, promoter analysis and gene structure determination was also performed. Half of the family members were located on chromosome 2 and the rest on chromosomes 1, 4, 5 and 6. Number of introns in the gene family members varied from 0 to 8. Totally, 19 sorts of cis elements including abiotic stress responsive elements were found in HvSnRK2s promoter sequences. Expression pattern of the family members were evaluated in different tissues, treatments and genotypes, based on the microarray data. Expression of HvSnRK2.6 was up-regulated by drought, salt and cold stresses implementing its important role in signal transduction of these stresses and tolerance induction to them. It is expected that this gene could be used in plant manipulation and breeding programs aimed for tolerance enhancement to abiotic stresses especially drought.

کلیدواژه‌ها [English]

  • Transcrption Factors
  • Abiotic stresses
  • Barley
  • SnRK2 family

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