بررسی گروه‌های کارکردی و تغییرات الگوی بیان ژن‌ها در گیاه C3 برنج و C4 ذرت تحت تنش خشکی با استفاده از تجزیه و تحلیل داده‌‌های ریزآرایه‌

نوع مقاله: علمی پژوهشی

نویسندگان

1 کارشناسی‌ارشد بیوتکنولوژی کشاورزی، دانشگاه پیام نور، تهران

2 دانشیار گروه بیوتکنولوژی، دانشکده کشاورزی، دانشگاه صنعتی اصفهان

چکیده

خشکی یکی از عمده‌ترین تنش‌های محیطی محسوب می‌شود که رشد و توسعه گیاهان را تا حد زیادی تحت تاثیر قرار می‌دهد. واکنش‌های گیاهان در برابر این تنش‌ با بروز تغییرات زیاد در شبکه‌های پیچیده حاوی تعداد زیادی ژن همراه است. در پژوهش حاضر تغییرات الگوی بیان ژن‌ها در دو ژنوتیپ حساس و متحمل گیاه برنج (به‌عنوان گیاه C3) و گیاه ذرت (به‌عنوان گیاه C4) با استفاده از آرایه‌های ژنوم ذرت شامل 734/ 17پروب‌ست و ژنوم برنج شامل 381/57 پروب‌ست مورد بررسی قرار گرفت. داده‌های ریزآرایه‌، جهت شناسایی ژن‌های درگیر در پاسخ به تنش در دو شرایط کنترل و تنش از بانک اطلاعاتی GEO/NCBI، گرفته شد. نتایج نشان داد که به ترتیب تعداد 1861 (49/10 درصد) و 1753 (8/9 درصد) ژن در ژنوتیپ حساس و متحمل ذرت و تعداد 9252 (16 درصد) و 7971 (8/13 درصد) ژن در ژنوتیپ حساس و متحمل برنج پس از تنش خشکی در سطح یک درصد تغییر بیان معنی‌داری داشتند. از این تعداد به ترتیب 1012 و 175 ژن در برگ ژنوتیپ متحمل و حساس برنج و ذرت افزایش بیان معنی‌داری نشان دادند. دیاگرام ون نشان داد که به ترتیب تعداد 663 و 158 ژن به ترتیب و به صورت مشترک در ارقام متحمل و حساس برنج و همچنین ذرت کاهش بیان معنی‌داری دارند. گیاه برنج (به‌عنوان گیاه C3) پنج برابر در مقایسه با گیاه ذرت (به‌عنوان گیاه C4) واکنش گسترده‌تری به تنش خشکی از خود نشان داد. گروه‌بندی کارکردی ژن‌های دارای افزایش بیان در دو گونه گیاهی مشخص کرد که در ذرت گروه کارکردی پروتئین‌های ریبوزومی و ‌فسفاتازها دارای بیشترین تعداد ژن هستند در حالیکه در برنج گروهای کارکردی اتصال به فلزات، پاسخ به تنش، پاسخ به محرک‌های زیستی و انتقال پیام بیشترین ژن‌ها را شامل شدند.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

The Study of Functional Groups and Changes in Genes Expression Pattern of Rice (C3) and Maize (C4) under Drought Stress using Microarray Data Analysis

نویسندگان [English]

  • ِSedigheh Akhtartavan 1
  • Majid Talebi 2
1 Department of Agricultural Biotechnology, Payame Noor University, Tehran, Iran
2 Department of Biotechnology, College of Agriculture, Isfahan University of Technology, Isfahan, Iran.
چکیده [English]

Drought is one of the major environmental stresses that greatly affects growth and development of plants. The plants reaction against this stress is associated with showing massive changes in complex gene networks. In the present study, the changes of gene expression patterns in two sensitive and tolerant genotypes of rice (as C3 plant) and maize (as C4 plant) were investigated using maize genome arrays containing 17,734 probe sets and the rice genome containing 57/381 probe set. The microarray data were taken from the GEO/NCBI database on both stress and control conditions to identify the genes involved in responses to the stress. The results indicated that 1861 (10.49%) and 1753 (8.9%) genes in the sensitive and tolerant maize, respectively, and 9252 (16%) and 7971 (13.8%) genes in the sensitive and tolerant rice, respectively, changed significantly after drought stress at the level of one percent. From of these genes, 1012 and 175 genes in the sensitive and tolerant genotype leaf of rice and maize, were significantly up-regulated, respectively. The Venn diagram showed that 663 genes of rice and 158 genes of maize, have significantly down-regulated. Rice plant, as a C3 plant, showed five times wider reaction to drought stress in compared with maize plant, as a C4 plant. The functional grouping of the up-regulated genes in two species revealed that functional group of ribosomal proteins and phosphatases in maize plant have the most abundant categories, whereas the functional groups of metal-binding, stress response, response to biological stimuli and signals in rice plant contained the highest percentage of the genes.

کلیدواژه‌ها [English]

  • Drought stress
  • Zea mays
  • Oryza sativa
  • Microarray analysis
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