تأثیر کاربرد سالیسیلیک‌اسید در القاء مقاومت گندم به بیماری سپتوریای برگی (STB) با عامل Zymoseptoria tritici

نوع مقاله: علمی پژوهشی

نویسندگان

1 دانشجوی دکتری بیماری‌شناسی گیاهی، دانشکده کشاورزی، دانشگاه تربیت مدرس، تهران

2 دانشیار گروه بیماری‌شناسی گیاهی، دانشکده کشاورزی، دانشگاه تربیت مدرس، تهران

3 دانشیار موسسه تحقیقات اصلاح و تهیه نهال و بذر، سازمان تحقیقات، آموزش و نرویج کشاورزی، کرج

4 استادیار پژوهشگاه ملی مهندسی ژنتیک و زیست فناوری، تهران

چکیده

این تحقیق به منظور بررسی تأثیر سالیسیلیک اسید روی کاهش شدت بیماری سپتوریای برگی گندم (STB) در سه رقم تتراپلوئید و سه رقم هگزاپلوئید، در شرایط کنترل شده در مرحله گیاهچه‌ای انجام گرفت. گیاهچه‌های 10 روزه گندم ابتدا با سالیسیلیک‌ اسید به غلظت‌های صفر (شاهد)، یک، دو و چهار میلی‌مولار با مه‌پاش دستی تیمار شدند. پس از گذشت 24 ساعت، گیاهچه‌ها با استفاده از سوسپانسیون اسپور قارچ به غلظت 107 اسپور بر میلی‌لیتر مایه‌زنی شدند. ارزیابی بیماری 21 روز پس از مایه‌زنی بر اساس تخمین درصد سطح برگی با لکه‌های بافت مرده حاوی پوشش پیکنیدی انجام گرفت. نتایج تجزیه واریانس داده‌ها نشان داد بین سطوح مختلف سالیسیلیک‌اسید از نظر کاهش شدت بیماری و تأثیر متقابل غلظت- رقم اختلاف معنی‌دار وجود دارد. به طوری که سالیسیلیک اسید درکاهش شدت بیماری در هر دو گروه ارقام تتراپلوئید و هگزاپلوئید مؤثر بوده و تیمار یک میلی‌مولار بیشترین تأثیر در کاهش شدت بیماری را نشان داد. در غلظت‌های دو و چهار میلی‌مولار کاهش شدت بیماری معنی دار بود اما میزان اثربخشی سالیسیلیک اسید در القاء مقاومت کمتر از غلظت یک میلی‌مولار بود. در مجموع این نتایج نشان داد که فعال کردن سیستم‌های دفاعی گیاه میزبان با استفاده از سالیسیلیک اسید می‌تواند نقش قابل توجهی در القاء مقاومت اکتسابی سیستمیک علیه بیماری سپتوریای برگی در گندم باشد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

The effect of salicylic acid application on the reduction of wheat septoria leaf blotch (STB) symptoms causing by Zymoseptoria tritici

نویسندگان [English]

  • Azadeh Ghaneie 1
  • Naser Safaie 2
  • Rahim Mehrabi 3
  • Forough Sanjarian 4
1 Ph.D. Candidate of Plant Pathology, respectively, Tarbiat Modares University, Tehran, Iran.
2 Associate Professor, respectively, Tarbiat Modares University, Tehran, Iran.
3 Associate Professor, Seed and Plant Improvement Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
4 Assistant Professor, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.
چکیده [English]

This study was carried out to evaluate the effect of salicylic acid on the reduction of disease severity of septoria tritici blotch (STB) in three tetraploeid and three hexaploeid wheat cultivars, under controlled conditions at seedling stage. The 10-day-old seedlings were initially treated with 0 (control), 1, 2 and 4 mM of salicylic acid with a handy sprayer. After 24 hrs, the seedlings were inoculated with fungal spore suspensions adjusted at 107 sopres/ml. The disease progress was evaluated 21 days post inoculation based on visual estimation of the percentage of leaf area with necrotic lesions bearing pycnidia. The results showed that there were significant differences on the reduction of disease severity among different concentrations of applied salicylic acid as well as among cultivar/ concentration interactions. In addition, the results showed that application of salicylic acid reduced disease severity of both tetraploid and hexaploid wheat cultivars and 1 mM salicylic acid was the most effective concentration on the reduction of disease severity. Although, the disease severity was significantly reduced by application of 2 and 4 mM salicylic acid, the effectiveness of these concentrations was less than application of 1mM. Overall, these results revealed that activation of plant defense systems through application of salicylic acid could play a significant role in systemic acquired resistance against STB disease.

کلیدواژه‌ها [English]

  • Zymoseptoria tritici
  • salicylic acid
  • Septoria leaf blotch
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