آنالیز شبکه‌ هم‌بیان ژنی وزن‌دار برای یافتن ماژول های تنظیم‌شونده توسط هورمون جاسمونیک‌اسید در آرابیدوپسیس

نوع مقاله: علمی پژوهشی

نویسندگان

1 دانشجوی دکتری، گروه زراعت و اصلاح‌نباتات، دانشکده کشاورزی، دانشگاه زنجان، زنجان

2 استادیار گروه زراعت و اصلاح‌نباتات، دانشکده کشاورزی، دانشگاه زنجان، زنجان

3 دانشیار گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه زنجان، زنجان

4 استادیار بخش زراعت، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان کرمانشاه، کرمانشاه

چکیده

شرایط محیطی منجر به ساخت ملکول‌های پیام‌رسان از جمله فیتوهورمون‌ها در گیاهان می‌گردد که وظایف مهمی بعنوان پیام‌رسان اولیه در مسیر ترارسانی و تنظیم فعالیت‌های سوخت و ساز سلول را بعهده دارند. هورمون جاسمونیک‌اسید با کنترل عامل‌های رونویسی در فرآیندهای نموی و پاسخ گیاهان ‌به تنش‌های مختلف نقشی کلیدی ایفا ‌می‌کند. با وجود مطالعات فراوان صورت گرفته، پاسخ‌ گیاهان به این هورمون به‌طور کامل شناخته نشده است. در اینجا، داده‌های ریزآرایه مربوط به گیاه آرابیدوپسیس از پایگاه داده GEO برای آنالیز شبکه همبیانی مورد استفاده قرار گرفت. از تجزیه تحلیل آن‌ها با روش WGCNA (شبکه‌ هم‌بیانی ژنی وزن‌دار)، 25 گروه‌ ژنی (ماژول‌) بدست آمد که پروفایل بیانی آنها در پاسخ به جاسمونیک‌اسید با یکدیگر دارای همبستگی معنی‌دار بالایی بود. برای بررسی معنی‌داربودن آماری هر ماژول از نظر زیستی از تجزیه و تحلیل هستی‌شناسی ژن استفاده شد. نتایج نشان داد که فرآیندهای زیادی از جمله فتوسنتز، مرگ برنامه‌ریزی‌ شده سلول و پاسخ به تنش‌های مختلف به وسیله ‌جاسمونات کنترل ‌شدند. علاوه‌ براین، تعداد زیادی عامل رونویسی، برای مثال 11 ژن از خانواده NAC و 12 ژن از خانواده bHLH، در تنظیم پاسخ‌های جاسمونیک‌اسید نقش داشتند و فرآیندهایی هم‌چون پاسخ به تنش‌های زیستی و غیر زیستی، نمو گل و پاسخ به نور را کنترل نمودند.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Weighted gene co-expression network analysis of regulatory modules by jasmonic acid in Arabidopsis

نویسندگان [English]

  • Maryam Mortezaeefar 1
  • Reza Fotovat 2
  • Farid Shekari 3
  • Shahryar Sasani 4
1 Ph.D. Candidate Student, Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Zanjan, Zanjan, Iran
2 Assistant Professor, Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Zanjan, Zanjan, Iran
3 Associate Professor, Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Zanjan,
4 Assistant Professor, Agricultural and Natural Resources Research Centre of Kermanshah, Kermansha, Iran
چکیده [English]

Environmental conditions lead to biosynthesis of signaling molecules including phytohormones in plants which have important functions as primary messengers in signal transduction and regulating cell metabolism. Jasmonic acid hormone by controlling the transcription factors can play key roles in response to various stresses and developmental processes in plants. Despite numerous studies, plant responses to the hormone are not completely understood. Here, microarray data of Arabidopsis from GEO database was used for analysis of co-expression network. WGCNA (Weighted Gene Co-expression Network Analysis) analysis determines 25 gene groups (modules) that their expression profiles correlated highly significant with each other in response to jasmonic acid. Gene ontology was utilized to investigate each module for statistical significance. This analysis indicated that jasmonic acid controls many processes including photosynthesis, cell programmed death, and response to various stresses. In addition, many of transcription factors such as 11 genes of NAC family and 12 genes of bHLH family play roles in the regulation of jasmonic acid responses and adjust processes including response to biotic and abiotic stresses, flower development and response to light.

کلیدواژه‌ها [English]

  • biotic and abiotic stress
  • Flowering
  • photosynthesis
  • programming cell death
  • system biology
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