بررسی گسترده ژنومی خانواده ژن‌های پاسخ‌گو به هورمون اکسین (ARF) در لوبیا

نوع مقاله: علمی پژوهشی

نویسندگان

1 کارشناسی‌ارشد بیوتکنولوژی کشاورزی، دانشگاه کردستان، سنندج، ایران

2 دانشجوی دکتری، دانشکده زمین و زندگی، مرکز تحقیقات تنوع، دانشگاه کاتولیک لوون، ب-1348 بلژیک

چکیده

اکسین نقش مهمی در تنظیم مراحل مختلف رشد و نمو گیاهان دارد. مطالعات نشان‌ داده است که اکسین این فرآیندها را با استفاده از ژن‌های پاسخ‌گو به اکسین (ARF) تنظیم می‌کند. با توجه به نوع دومین میانی، پروتئین ARF به عنوان یک فاکتور رونویسی با اتصال به ژن‌های هدف باعث سرکوب و یا تحریک بیان این ژن‌ها می‌شود. ژن‌های ARF در چند گونه مختلف گیاهی با استفاده از روش‌های مولکولی مورد مطالعه قرار گرفته‌اند، با این حال برای درک بهتر مکانیسم این پروتئین‌ها نیاز به مطالعات بیشتری در گیاهان است. در این مطالعه با استفاده از روش‌های محاسباتی 27 ژن ARF شناسایی شد که در قسمت‌های مختلف کروموزوم‌های لوبیا پراکنده هستند. بررسی‌ درخت فیلوژنتیکی نشان داد این توالی‌ها در چهار گروه مجزا قرار دارند بدون اینکه در بین این توالی‌ها مضاعف‌شدگی مشاهده شود. بیان ژن‌های ARF باتوجه به نوع ژن و بر اساس نوع بافت به ترتیب به پنج و دو گروه تقسیم شد. ژن‌های ARF در بافت‌های برگ، سه برگچه‌های جوان، ساقه، غلاف‌های رسیده و غلاف‌های جوان در مقایسه با سایر بافت‌های دیگر بیان بیشتری داشتند. از کل توالی‌های ARF مورد بررسی، تعداد 9 و 17 توالی به ترتیب به عنوان محرک و سرکوب‌کننده بیان ژن شناسایی شدند.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Genome-wide analysis of the auxin response factor (ARF) gene family in Phaseolus vulgaris

نویسندگان [English]

  • Sohaila Fatemi 1
  • Khaled Mirzaei 2
  • Mohamed Sedigh Kamangar 1
1 M.Sc. of Biotechnology in Agriculture, University of Kurdistan, Sanandaj, Iran
2 Ph.D. Student, Earth & Life Institute, Biodiversity Research Centre, Université Catholique de Louvain (UCL), B-1348 Belgium
چکیده [English]

Auxin has an important role in the various stages of plants growth and development. Studies have shown that auxin regulates these processes by auxin responsefactors (ARF) family. ARF proteins due to their central domain, mediate the cellular response to the auxin by activating or repressing the expression of downstream genes. ARF genes in several different plant species have been studied by molecular methods however to better understand the mechanisms of these proteins more studies are needed. In this study with computational methods, we found 27 ARF proteins, which they were scattered on the different parts of Phaseolus vulgaris chromosomes. The phylogenetic analysis of these sequences revealed they were divided into four different classes without any sign of gene-duplication between them. ARF genes expression according to the types of gene and based on the types of tissue respectively showed that they group into the five and two classes. In comparing with other tissues, ARF genes in leaf, young trifoliates, stems, mature pods and young pods tissues have a high level of expression. Of the 27 ARF Phaseolus vulgaris sequences surveyed in this study, nine ARF proteins were gene activator and 17 ARF proteins were gene suppressor.

کلیدواژه‌ها [English]

  • Phaseolus vulgaris
  • Gene family
  • auxin
  • ARF
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