بررسی روابط بین ژنوم‌های D، S و U جنس گندم‌نیای وحشی (Aegilops) با ژنوم A گندم با استفاده از نشانگر مولکولی ISSR

نوع مقاله: علمی پژوهشی

نویسندگان

1 دانش آموخته کارشناسی ارشد، اصلاح نباتات، دانشکده کشاورزی، دانشگاه ایلام، ایران

2 دانشیار، اصلاح نباتات، دانشکده کشاورزی، دانشگاه ایلام، ایران

3 3. دکترای اصلاح نباتات بخش تحقیقات جنگل‌ها و مراتع، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان کرمانشاه، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرمانشاه، ایران

چکیده

هدف از تحقیق حاضر بررسی روابط بین ژنوم‌های D، S و U در سه گونه‌ی دیپلوئید از جنس گندم‌نیای وحشی (Aegilops) با ژنوم A در دو گونه‌ی دیپلوئید از جنس گندم (Triricum) با استفاده از 15 آغازگر ISSR می‌باشد. آغازگرهای ISSR توانستند 105 مکان را شناسایی کنند، که 6 مکان یک شکل و 99 مکان چندشکل بودند. میانگین تعداد مکان تولید شده 7 بود و متوسط تعداد مکان چند شکل 60/6 بود. آغازگرهای IS13، IS6 وUBC865 به عنوان آغازگرهای برتر در شناسایی تنوع ژنتیکی در بین جمعیت‌های مورد بررسی معرفی شدند. در بین گونه‌های هر دو جنس تنوع ژنتیکی بالایی بر اساس نشانگر مورد استفاده وجود داشت و سهم بالای واریانس بین گونه‌ای نسبت به واریانس درون گونه‌ای نشان داد که گونه‌ها بر اساس نشانگر مورد بررسی کاملاً تفرق داشتند. نتایج ماتریس تشابه، تجزیه خوشه‌ای و تجزیه به مختصات اصلی نشان داد که دو جنس از هم تفکیک شده‌اند و در گونه‌های جنس گندم‌نیای وحشی گونه Ae. umbellulata (ژنوم U) بیشترین فاصله را با دیگر گونه‌ها داشت و دو گونه Ae. strangulate و Ae. tauschii (ژنوم D) کمترین فاصله را داشتند و در مرحله بعد با گونه Ae. speltoides (ژنوم S) هم گروه شدند. بنابراین ژنوم D فاصله کمتری با ژنوم S نشان داد و ژنوم U بیشترین فاصله را با دو ژنوم دیگر براساس نشانگر مورد بررسی داشت. ژنوم A متعلق به جنس گندم نیز بیشترین فاصله را با سه ژنوم جنس گندم‌نیای وحشی داشت. اما در بین ژنوم‌های متعلق به جنس گندم‌نیای وحشی، ژنوم U دارای فاصله کمتری با ژنوم A بود.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Evaluation of relationships of D, S and U genomes of Aegilops genus with A genome of Triticum using ISSR molecular marker

نویسندگان [English]

  • Lida Feridooni 1
  • Ali Ashraf Mehrabi 2
  • Hooshmand Safari 3
1 Former M.Sc. Student, Plant Breeding, College of Agriculture, Ilam University, Iran,
2 Associate Professor, Plant Breeding, College of Agriculture, Ilam University, Iran
3 Ph.D. Department of Forests and Rangelands, Kermanshah Agricultural and Natural Resources Research and Education Center, Kermanshah, Iran
چکیده [English]

The objective of this study was to investigate the relationships between D, S, and U genomes in three diploid species of Aegilops genus with genome A in two diploid species of Triticum genus, using 15 ISSR primers. The ISSR primers were able to identify 105 locations, that the number of 6 locations were monomorphic and 99 locations were polymorph. The mean number of generated location was 7.00 and the average number of polymorphic location was 6.60. The primers of IS13, IS6 and UBC865 were introduced as superior primers for determining of genetic variation in the studying populations. There was a high genetic diversity based on the used marker among the species of two genera and the high contribution of variance to interspecies than intraspecific showed that the species were completely differentiating according to the studied marker. The results of similarity matrix, cluster analysis and principal coordinate analysis were showed that the two species were separated from together and in the species of Aegilops genus, Ae. umbellulata (U genome) had the most distance with other species, Ae. strangulate and Ae. tauschii (D genome) had the lowest distance and falling in one group with Ae. speltoides (S genome) in the next stage. Therefore, the D genome showed a lower distance with the S genome and the U genome had the greatest distance with two other genomes based on studied markers. The A genome (Triticum genome) had the most distance with three Aegilops genome. But the U genome among genomes belonging to the Aegilops genus had a lower distance than A genome.

کلیدواژه‌ها [English]

  • Aegilops
  • ISSR Marker
  • Phylogenetic
  • Genomic reletionship
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