شناسایی ژن‌های کلیدی دخیل در تحمل به تنش شوری در گندم با استفاده از آنالیز داده‌های ریزآرایه

نوع مقاله: علمی پژوهشی

نویسندگان

1 دانشجوی دکتری بیوتکنولوژی کشاورزی، دانشکده کشاورزی، دانشگاه لرستان، خرم آباد، ایران

2 استادیار گروه زیست شناسی سیستم ها، پژوهشگاه بیوتکنولوژی کشاورزی ایران، سازمان تحقیقات، آموزش وترویج کشاورزی، کرج، ایران

3 دانشیار گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه لرستان، خرم آباد، ایران

4 استاد گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه لرستان، خرم آباد، ایران

5 استادیار گروه زیست‌شناسی سیستم‌ها، پژوهشگاه بیوتکنولوژی کشاورزی ایران، سازمان تحقیقات، آموزش وترویج کشاورزی، کرج، ایران

چکیده

تنش شوری یکی از مهمترین عوامل محدود‌کننده محیطی در تولید گندم می‌باشد و تحقیقات در راستای ایجاد ارقام متحمل به تنش شوری از اهمیت فوق‌العاده‌ای برخوردار است. شناسایی ژن‌ها و سازوکارهای دخیل در تحمل به شوری در راستای اصلاح مولکولی این گیاه برای تحمل به شوری ضروری است. در این تحقیق به منظور شناسایی ژن‌های پاسخ-دهنده به تنش شوری در گندم، دو سری داده ریزآرایه مرتبط با تنش شوری گندم از پایگاه داده NCBI مورد آنالیز قرار گرفتند. نتیجه این تجزیه و تحلیل، شناسایی 3096 و 2060 ژن پاسخ‌دهنده به تنش شوری به ترتیب در ریشه و اندام هوایی بود. نتایج هستی‌شناسی (Ontology) ژن-های افتراقی در هر دو بافت نشان داد که این ژن‌ها در بخش فرایندهای زیستی برای پاسخ به محرک‌های شیمیایی، پاسخ به تنش اکسیداتیو، انتقال، تنظیم رونویسی و پردازش متالبولیکی کربوهیدرات‌ها و در بخش عملکرد مولکولی برای فعالیت کاتالیتیکی، فعالیت اتصال و فعالیت اکسیدوردوکتازی دارای فراوانی بالای معنی‌داری بودند. همچنین، به منظور تعیین ژن‌های کلیدی در ایجاد تحمل به شوری، ژن‌های پاسخ‌ دهنده در ریشه تحت آنالیز هاب قرار گرفتند. بر اساس نتایج به‌دست آمده، نقش ژن‌های تنظیم‌کننده‌ای مانند پروتئین کینازها، پروتئین فسفاتازها و عوامل رونویسی همانند MYB و WRKY در ایجاد تحمل به تنش شوری مورد تاکید قرار گرفت.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Identification of the key salt tolerance involved genes in wheat using microarray data analysis

نویسندگان [English]

  • Nazanin Amirbakhtiar 1
  • Zahra-Sadat Shobbar 2
  • Ahmad Ismaili 3
  • Farhad Nazarian Firouzabadi 4
  • Mohammad reza Ghaffari 5
1 Ph.D. Student, Department of Agronomy and Plant Breeding, Faculty of Agriculture, Lorestan University, Khorramabad, IranKhorramabad, Lorestan
2 Assistant Professor, Agricultural Biotechnology Research Institute of Iran, Department of Systems Biology, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
3 Associate Professor, Department of Agronomy and Plant Breeding, Faculty of Agriculture, Lorestan University, Khorramabad, Iran
4 Professor, Department of Agronomy and Plant Breeding, Faculty of Agriculture, Lorestan University, Khorramabad, Iran
5 Assistant Professor, Agricultural Biotechnology Research Institute of Iran, Department of Systems Biology, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
چکیده [English]

Salt stress is considered as one of the most important constraints in wheat production worldwide, thus for, research toward development of tolerant varieties is of great importance. Discovering genes and molecular mechanisms involved in salt tolerance are the primary steps in molecular breeding for salinity. In this study, taking advantage of the data deposited in NCBI Gene Bank, two salinity-related microarray data sets of bread wheat were analyzed to identify salt responsive genes. Bioinformatics’ analyses indicated that 3096 and 2060 genes were salt responsive genes in root and shoot, respectively. Gene ontology analysis of salt responsive genes showed that these genes were enriched for response to chemical stimulus, response to oxidative stress, transport, regulation of transcription and carbohydrate metabolic process in biological process category in both tissues. Furthermore, the differentially expressed genes in metabolic process category were enriched for catalytic activity, binding and oxidoreductase activity in both tissues. In order to determine the key genes involved in salt tolerance, hub analysis was performed on the salt responsive genes identified in the root. Based on the achieved results, the role of regulatory genes including protein kinases, protein phosphatases and transcription factors such as MYB and WRKY, was highlighted in inducing salt tolerance.

کلیدواژه‌ها [English]

  • Triticum aestivum
  • Salt stress
  • Microarray data
  • Hub analysis
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