برهمکنش باکتریهای Bacillus amyloliquefaciens و Azospirillum oryzae در تحریک رشد گیاه گندم و کاهش بیماری زایی قارچ Fusarium graminearum

نوع مقاله: علمی پژوهشی

نویسندگان

1 دانشجوی دکتری، گروه گیاهپزشکی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج، ایران

2 استاد، گروه گیاهپزشکی پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج، ایران

3 استاد، بخش تحقیقات بیوتکنولوژی میکروبی، پژوهشگاه بیوتکنولوژی کشاورزی ایران (ABRII)، سازمان تحقیقات، آموزش و ترویج کشاورزی (AREEO)

چکیده

تلقیح توام ریزوباکتری‌های افزاینده رشد گیاه و عوامل کنترل زیستی آفات و بیماری‌ها، رویکردی در مدیریت سلامت و بهبود تولید و کیفیت محصولات کشاورزی می‌باشد. در این تحقیق به مطالعه اثرات متقابل دو سویه باکتریایی Bacillus amyloliquefaciens UTB96 و Azospirillum oryzae NBT506 در تحریک رشد گیاه گندم و بازدارندگی قارچ Fusarium graminearum (عامل بلایت خوشه و پوسیدگی طوقه گندم) در دو حالت کشت منفرد و هم‌کشتی پرداخته شد. نتایج نشان داد که باکتری‌ها در کشت منفرد و هم‌کشتی بخوبی از رشد قارچ بیماری‌زا به‌صورت مستقیم و با ترکیبات فرار جلوگیری کردند و این نتایج در شرایط اتاقک رشد هم مشاهده شد که هم‌کشتی دو سویه به شدت باعث افزایش شاخص‌های رشدی گیاه و کاهش خسارت و علائم بیماری نسبت به شاهد شد. بهترین تیمارهای باکتری‌ها در حالت محلول‌پاشی در سطح خاک بودند. در محلول‌پاشی سطح خاک با این عوامل میکروبی، طول ریشه 80-14 درصد، وزن تر ریشه 167-18 درصد، وزن خشک ریشه 110-4 درصد، طول ساقه 61-17 درصد، وزن تر ساقه 169-47 درصد و وزن خشک ساقه 90-4/0 درصد افزایش را نشان دادند همچنین درصد کاهش شاخص بیماری نیز 100- 62 درصد در تیمارهای مختلف برآورد شد. به‌طور کلی هم‌کشتی دو باکتری اثرات هم‌افزایی در افزایش رشد گندم و کاهش رشد قارچ بیمارگر نشان داد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Interaction of Bacillus amyloliquefaciens and Azospirillum oryzae on wheat growth promotion and Fusarium graminearum disease inhibition

نویسندگان [English]

  • Negar Bagheri 1
  • Masoud Ahmadzadeh 2
  • Gholamreza Salehi Jouzani 3
1 Ph.D. Candidate, Department of Plant Protection, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
2 Professor of Department of Plant Protection, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
3 Professor of Microbial Biotechnology Department, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
چکیده [English]

Co-inoculation of plant growth promoting bacteria and biological control agents, is a strategy to improve health, yield and quality of crop production. The objective of the present study was to evaluate interactions of two bacterial strains, Bacillus amyloliquefaciens UTB96 and Azospirillum oryzae NBT506, on growth promotion of wheat and control of the causal agent of Fusarium head blight, Fusarium graminearum. The results showed that single and co-culture of these strains inhibit mycelium growth of F. graminearum by direct inhibition and volatile organic compounds. In germinator assays, different wheat growth features were increased and disease index was decreased. Results showed that co-inoculation or single application of the bacteria in the soil significantly enhanced root length (14-80%), root fresh weight (18-167%), root dry weight (4-110%,), shoot length (17-61%), shoot fresh weight (47-169%) and shoot dry weight (up to 90%). In addition, a significant decrease in disease index (62-100%) was observed in different single and co-culture treatments. In conclusion, the studied two bacterial strains showed synergistic effects on wheat growth promotion and fungal growth inhibition.

کلیدواژه‌ها [English]

  • Azospirillum oryzae
  • Bacillus amyloliquefaciens
  • Biological control
  • Interaction
  • Synergistic effect
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