مطالعه فیلوژنتیکی و ساختاری پلی‌آمین اکسیدازهای گیاهی

نوع مقاله : علمی پژوهشی

نویسندگان

1 دانشجو دکتری گروه علوم و زیست‌فناوری گیاهی، دانشکده علوم و فناوری زیستی، دانشگاه شهید بهشتی، تهران، ایران

2 استاد گروه علوم و زیست‌فناوری گیاهی، دانشکده علوم و فناوری زیستی، دانشگاه شهید بهشتی، تهران، ایران

چکیده

محتوای پلی‌آمین‌های درون سلولی نه تنها توسط بیوسنتز و انتقال بلکه توسط کاتابولیسم آن‌ها به وسیله پلی‌آمین اکسیدازهای (PAO) وابسته به فلاوین آدنین دی‌نوکلئوتید (FAD) تنظیم می‌شود. نتایج حاصل از مطالعات مختلف در مورد پروتئین‌‌های PAO در فرایندهای مختلف نموی و پاسخ به تنش‌های محیطی تأیید کننده اهمیت این پروتئین در زندگی گیاهی می‌باشد، با این حال مطالعه جامعی در مورد روابط فیلوژنتیکی و ساختاری PAOs گیاهی وجود ندارد. در این مطالعه آنالیزهای بیوانفورماتیکی به منظور درک بهتر روابط فیلوژنتیکی و ساختاری بر روی 58 توالی پروتئینی PAO از 15 گونه مختلف گیاهی انجام شد. کلاسترهای چندگانه با دو برابر شدگی ژنی هم در گونه‌های تک‌لپه‌ای و هم در گونه‌های دو لپه‌ای شناسایی شد. بر اساس موتیف‌های حفاظت شده به‌دست آمده توسط ابزارهای MEME و MAST، چهار موتیف در بیشتر گونه‌های گیاهی یکسان بودند. آنالیزهای ساختاری بر روی PAOs مربوط به Oryza sativa و Arabidopsis thaliana به عنوان نماینده گیاهان تک‌لپه‌ای و دو لپه‌ای که اطلاعات ساختاری آن‌ها در دسترس نبود، انجام شد. آنالیز ساختار دوم نشان داد که مارپیچ آلفا در میان عناصر ساختار دوم غالب بوده و پس از آن به‌ترتیب راندوم کویل، صفحات بتا و دور بتا برای تمامی توالی‌ها دارای بیشترین مقدار بود. پیش‌بینی ساختار سوم توسط سرور SWISS-MODEL انجام شد. ساختمان این توالی‌ها دارای دو دومین مشترک بود. این اولین مطالعه در مورد روابط فیلوژنتیکی و ساختاری PAOs گیاهی است و این نتایج ممکن است مبنای نظری برای مطالعات آتی در مورد جزئیات ساختمانی و عملکردی PAOs گیاهی فراهم کند.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Structural and functional studies of plant Polyamine Oxidase

نویسندگان [English]

  • Mohsen Hosseini 1
  • Abbas Saidi 2
1 Ph.D. Candidate, Department of Plant Sciences and Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, G.C, Tehran, Iran.
2 Professor, Department of Plant Sciences and Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, G.C, Tehran, Iran.
چکیده [English]

Intracellular polyamine contents are regulated not only by biosynthesis and transport but also by catabolism through FAD dependent polyamine oxidases (PAOs). The results of various studies on PAO proteins in developmental processes and response to environmental stresses confirm the importance of this protein in plant life; however, there is no comprehensive study of phylogenetic and structural relationships of plant PAOs. In the present study, to better understand phylogenetic and structural relationships of PAO proteins, bioinformatics analyses were performed on 58 PAO protein sequences of 15 different plant species. Multiple clusters with gene duplications were identified in both dicot and monocot-species. According to the conserved motifs obtained by MEME and MAST tools, four motifs were similar in most plant species. As there is no structural information available on PAOs, structural analyses were carried out on PAOs from Oryza sativa and Arabidopsis thaliana as representative of monocot and dicot plants, respectively. Secondary structure analysis revealed that alpha helix dominated among secondary structure elements followed by random coils, extended strand and beta turns for all sequences. Tertiary structures were predicted with SWISS-MODEL server. The best templates with high similarity that their structure determined by experimental methods were selected. To our knowledge, this is the first report of phylogenetic and structural relationships of plant PAOs. Our results may provide a theoretical basis for future studies of functional and structural details of plants PAOs.

کلیدواژه‌ها [English]

  • Bioinformatics analysis
  • Phylogenetic
  • Polyamine
  • Polyamine oxidases

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