طراحی و ساخت سازه دو ژنی حاوی ژن EPSPS و 11 kDa delta zein به منظور تراریختی سویا با هدف بهبود محتوی متیونین و تحمل به علف‌کش گلایفوسیت

نوع مقاله: علمی پژوهشی

نویسندگان

1 گروه اصلاح و بیوتکنولوژی کشاورزی، گروه اصلاح و بیوتکنولوژی کشاورزی، دانشگاه زابل، زابل، ایران

2 پژوهشگاه بیوتکنولوژی کشاورزی، سازمان تحقیقات، ترویج و آموزش کشاورزی

3 پژوهشگاه بیوتکنولوژی کشاورزی سازمان تحقیقات، ترویج و آموزش کشاورزی، کرج، تهران

10.30473/cb.2019.46927.1776

چکیده

سویا یک منبع ممتاز پروتئین در تغذیه انسان و سایر حیوانات به شمار می‌رود و به منظور تولید و بهره برداری اقتصادی از روغن و پروتئین آن کشت می‌شود. این گیاه همانند سایر اعضای خانواده لگومینه، حاوی مقادیر کم اسید‌های آمینه گوگرد‌دار (متیونین و سیستئین) است. استفاده از یک نشانگر انتخابی مناسب، در باززایی گیاهان حاصل از انتقال ژن و همچنین افزایش نرخ انتقال ژن مؤثر خواهد بود. گلایفوسیت به‌عنوان یک علف‌کش غیر‌انتخابی برای کنترل دامنه وسیعی از علف‌های هرز در دنیا استفاده می‌شود. این پژوهش با هدف ساخت سازه دوژنی به منظور انتقال همزمان ژن‌های EPSPS و 11 kDa delta zein به سویا با استفاده از روش اگروباکتریوم به منظور تحمل به علف‌کش گلایفوسیت و بهبود محتوای اسید‌آمینه متیونین انجام شده است. پس از انجام مراحل کشت بافت، انتقال ژن و باززایی، گیاهان حاصل از انتقال ژن در نسل اول غلظت 5/3 میلی مولار علف‌کش گلایفوسیت را تحمل کردند. علاوه بر آن سنجش میزان شیکمیک اسید و کلروفیل در گیاهان حاصل نشان داد که این دو شاخص‌ بعد از تیمار گلایفوسیت به‌طور معنی‌داری در لاین‌های حاصل از انتقال ژن در مقایسه با شاهد تغییر می‌کند. آنالیزهای تکمیلی در حال انجام است.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Design and construction of two-genes construct consists of 11 kDa delta zein and EPSPS genes in order to transform soybean to improve the methionine content and induce resistance to glyphosate herbicide

نویسندگان [English]

  • Elham Saboori-Robat 1
  • Mahmood Solouki 1
  • Ali Akbar Habashi 2
  • Motahhareh Moshenpour 3
  • Abbasali Emamjomeh 1
1 Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Zabol, Zabol, Iran.
2 Department of Gene Transformation, Agriculture Biotechnology Research Institute of Iran (ABRII), Karaj, Iran.
3 Department of Gene Transformtion, Agriculture Biotechnology Research Institute of Iran (ABRII), Karaj, Iran
چکیده [English]

Soybean is considered as one of the best source of protein for the nutrition of humans and mammals, and also is cultivated as an economic source of both vegetable oil and protein. Soybean like other Leguminosae, contains low levels of S-amino acids (methionine and cysteine). Using an appropriate selectable marker can be effective in the regeneration of transgenic plants and increasing gene transfer rate. Glyphosate is a widely used non-selective herbicide with broad spectrum of weed control around the world. The aim of this study is constructing of two-genes construct consists of 11 kDa delta zein and EPSPS genes to improve the methionine content and induce resistance to glyphosate herbicide using Agrobacterium-mediated method in soybean. After experimental processes tissue culture, gene transformation and regeneration, plants produced by gene transformation showed glyphosate resistance at 3.5 mM concentration of glyphosate herbicide. Chlorophyll and shikimic acid content analysis also revealed that these two indexes in lines produce by gene transformation compared to wild type were significantly altered after glyphosate application. Complementary analyses are under progress.

کلیدواژه‌ها [English]

  • EPSPS
  • 11 kDa delta zein
  • shikimic acid
  • Glyphosate
  • methionine
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