با همکاری مشترک دانشگاه پیام نور و انجمن بیوتکنولوژی جمهوری اسلامی ایران

نوع مقاله : علمی پژوهشی

نویسندگان

1 دانشیار ، مرکز علم و فناوری زیست شناسی، دانشکده علوم پایه، دانشگاه جامع امام حسین(ع)، تهران، ایران

2 دانشجوی مقطع دکتری ، مرکز علم و فناوری زیست‌شناسی، دانشکده علوم پایه، دانشگاه جامع امام حسین(ع)، تهران، ایران

3 دانشجوی مقطع دکتری، گروه بیوتکنولوژی کشاورزی، دانشکده کشاورزی و منابع طبیعی، دانشگاه بین‌المللی امام خمینی(ره) قزوین، ایران

4 استاد گروه بیوتکنولوژی دانشگاه پیام نور، تهران، ایران.

5 دانشجوی کارشناسی‌ارشد دانشگاه پیام نور، تهران، ایران.

چکیده

غاسول صابونی (Saponaria officinalis)، یک گیاه چند ساله از خانوده میخک است. سیستم کشت ریشه‌های موئین جایگزین مناسبی جهت بهبود تولید ترکیبات با ارزش دارویی می‌باشد. کاربرد گسترده فناوری نانو در کشت ریشه‌های موئین، بستری مناسب جهت تولید عناصر فعال می‌باشد. در این مطالعه، تأثیر غلظت‌های مختلف نانوذرات تیتانیوم دی‌اکسید (0، 10، 20، 30 و 50 میلی‌گرم بر لیتر) در زمان‌های (24 و 48 ساعت) بر میزان رشد، ظرفیت آنتی‌اکسیدانی، محتوای فنول و فلاونوئید کل، فعالیت آنزیم‌های آنتی‌اکسیدانی، برخی از ترکیبات پلی‌فنول و میزان پروتئین SO6 در ریشه‌های موئین غاسول صابونی بررسی شد. بیشترین و کمترین میزان وزن تر ریشه‌های موئین (به ترتیب 09/3 و 96/0 گرم) به ترتیب در محیط کشت‌های تیمار شده با 10 و 20 میلی‌گرم بر لیتر نانوذرات تیتانیوم دی‌اکسید و مدت زمان تیمار 48 و 24 ساعت مشاهده گردید. بیشترین میزان فنول کل (79/9 میلی‌گرم گالیک اسید بر گرم وزن تر) و فلاونوئید کل (06/1 میلی‌گرم کوئرستین بر گرم وزن تر) در ریشه‌های موئین تیمار شده با غلظت‌های 50 و 30 میلی‌گرم بر لیتر نانوذرات تیتانیوم دی‌اکسید و به ترتیب مدت زمان تیمار 24 و 48 ساعت به‌دست آمد. بیشترین میزان اکثر پلی‌فنول‌ها از جمله رزمارینیک اسید، سینامیک اسید و روتین در مدت زمان تیمار 24 ساعت تولید شد. بیشترین میزان پروتئین SO6 در غلظت 50 میلی‌گرم بر لیتر و مدت زمان 48 ساعت به‌دست آمد. با بهینه‌سازی آزمایشگاهی تولید ریشه مویین گیاه غاسول صابونی، می توان SO6 را در فرمانتور تولید نمود.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Effect of titanium dioxide nanoparticles (TiO2NPs) application on the polyphenolic, phytochemical and SO6 protein compounds content in Saponaria officinalis hairy roots

نویسندگان [English]

  • Hosein Honari 1
  • Ahad Hedayati 2
  • Zahra Moshiri 3
  • Mohammad Ali Ebrahimi 4
  • Afsaneh Manouchehri 5

1 Associate Professor, Biology Science and Technology Center, Faculty of Basic Sciences, Imam Hossein University, Tehran, Iran

2 Ph.D. Student, Biology Science and Technology Center, Faculty of Basic Sciences, Imam Hossein University, Tehran, Iran

3 Ph.D. Student, Department of Agricultural Biotechnology, Faculty of Agriculture and Natural Resources, Imam Khomeini International University, Qazvin, Iran

4 Professor , Student of Biotechnology, Payame Noor University, Tehran, Iran.

5 M.Sc. Student of Biotechnology, Payame Noor University, Tehran, Iran.

چکیده [English]

Saponaria officinalis is a perennial plant of the clove family. Hairy root culture system is a suitable alternative to improve the production of compounds with medicinal value. Extensive application of nanotechnology in hairy root culture is a suitable substrate for the production of active elements. In this study, the effect of different concentrations of titanium dioxide nanoparticles (0, 10, 20, 30 and 50 mg / l) and two exposure times (24 and 48 hours) on growth rate, antioxidant capacity, phenol and flavonoid content, antioxidant enzymes activity, polyphenol compounds content and the amount of SO6 protein in soapy hairy roots were investigated. The highest and lowest fresh weight of hairy roots (3.09 and 0.96 g, respectively) were found in culture medium treated with 10 and 20 mg / l titanium dioxide nanoparticles at 48 and 24 hours of exposure time, respectively. The highest total Phenol (9.79 mg GAE per g FW) and flavonoid (1.06 mg Que per g FW) content were observed in hairy roots treated with 50 and 30 mg / l titanium dioxide nanoparticles at 24 and 48 hours of exposure time respectively. The highest amount of most polyphenols including rosmarinic acid, cinnamic acid and rutin were achieved during 24 hours of treatment. The highest levels of SO6 protein was obtained in hairy roots treated with 50 mg/l titanium dioxide nanoparticles after 48 hours. By laboratory optimization of hairy root production of soap plant, SO6 can be produced in fermenter.

کلیدواژه‌ها [English]

  • Soap dipper
  • capillary root
  • titanium dioxide and SO6 protein
Aebi H, Bergmeyer H (1974) Methods of enzymatic analysis. Academic Press, New York. 2: 674-684.
Boerjan W, Ralph J, Baucher M (2003) Lignin biosynthesis. Annual review of plant biology. 54(1): 519-546.
Chilton MD, Tepfer DA, Petit A, David C, Casse-Delbart F, Tempé J (1982) Agrobacterium rhizogenes inserts T-DNA into the genomes of the host plant root cells. Nature, 295(5848): 432-445.
Chiou A, Karathanos VT, Mylona A, Salta FN, Preventi F, Andrikopoulos NK (2007) Currants (Vitis vinifera L.) content of simple phenolics and antioxidant activity. Food chemistry. 102: 516-522.
Chung IM, Thiruvengadam M, Rekha K, Rajakumar G (2016) Elicitation enhanced the production of phenolic compounds and biological activities in hairy root cultures of bitter melon (Momordica charantia L.). Brazilian Archives of Biology and Technology. 59: 1-10.
Ebrahimi A, Galavi, M., Ramroudi, M., and Moaveni, P. (2016). Effect of TiO2 nanoparticles on antioxidant enzymes activity and biochemical biomarkers in Pinto Bean (Phaseolus vulgaris L.). Journal of Molecular Biology Research, 6(1), 58-66.
Falck GCM, Lindberg HK, Suhonen S, Vippola M, Vanhala E, Catalan J, Savolainen K, Norppa, H (2009) Genotoxic effects of nanosized and fine TiO2. Human and Experimental Toxicology 28(6-7): 339-352.
Fattahi M, Nazeri V, Torras-Claveria L, Sefidkon F, Cusido R, Zamani Z, Palazon J (2013) A new biotechnological source of rosmarinic acid and surface flavonoids: hairy root cultures of Dracocephalum kotschyi Boiss. Ind. Crops. Prod. 50: 256-263.
Frazier TP, Burklew CE, Zhang B (2014) Titanium dioxide nanoparticles affect the growth and microRNA expression of tobacco (Nicotiana tabacum). Functional and Integrative Genomics. 14 (1): 75-83.
Furukawa S, Fujita T (2017) Increased oxidative stress in obesity and its impact on metabolic syndrome. The Journal of Clinical Investigation. 114 (12): 1752-1761.
Ghorbanpour M (2015) Major essential oil constituents, total phenolics and flavonoids content and antioxidant activity of Salvia officinalis plant in response to nano-titanium dioxide. Indian Journal of Plant Physiology. 20(3): 249-256.
Ghorbanpour M, Hatami M, Hatami M (2015) Activating antioxidant enzymes, hyoscyamine and scopolamine biosynthesis of Hyoscyamus niger L. plants with nano-sized titanium dioxide and bulk application. Acta agriculturae Slovenica. 105 (1): 23-32.
Goran M, Marija D, Vesna P, Gordana S, Snežana Č (2018) Phytochemical analysis of Saponaria officinalis L. shoots and flowers essential oils. Natural Product research. 32(3): 331-334.
Guillon S, Trémouillaux-Guiller J, Pati PK, Rideau M, Gantet P (2006) Hairy root research: recent scenario and exciting prospects. Current Opinion in Plant Biology. 9 (3): 341-346.
Gülcin I (2012) Antioxidant activity of food constituents: an overview. Archives of Toxicology. 86(3):345-391.
Guy C, Haskell D, Neven L, Klein P, Smelser C (1992) Hydration-state-responsive proteins link cold and drought stress in spinach. Planta. 188: 265-270.
Hajimahdipour H, Khanavi M, SHekarchi M, Abedi Z, Pirali Hamedani M (2009) Study the best method of extraction of phenolic compounds in Echinacea purpurea. Journal of Medicinal Plants: 4 (8): 145-152.
Hasanlu T, Rezazadeh S, Rahnama H (2008) Hairy roots sources for the production of valuable pharmaceutical compounds. Journal of Medicinal Plants. 29: 34-42.
Hedayati A, Hosseini B, Palazon J, Maleki R (2020) Improved tropane alkaloid production and changes in gene expression in hairy root cultures of two Hyoscyamus species elicited by silicon dioxide nanoparticles. Plant Physiology and Biochemistry. 155: 416-428. ‏
Hu ZB, Du M (2006) Hairy root and its application in plant genetic engineering. Journal of Integrative Plant Biology. 48: 121-127.
Jiang P, Burczynski F, Campbell C, Pierce G, Austria JA, Briggs CJ (2007) Rutin and flavonoid contents in three buckwheat species Fagopyrum esculentum, F. tataricum, and F. homotropicum and their protective effects against lipid peroxidation. Food Research International. 40 (3): 356-364.
Kamalizadeh M, Bihamta M, Zarei A (2019) Drought stress and TiO2 nanoparticles affect the composition of different active compounds in the Moldavian dragonhead plant. Acta Physiologiae Plantarum. 41 (2): 21-29.
Kamalizadeh M, Bihamta MR, Peyghambari SJH (2014) Expression of Genes Involved in Rosmarinic Acid Biosynthesis Pathway in Dragonhead Affected by Nanoparticles. Genetics in the third millennium. 12: 3428-3437.
Karimi L, Mirjalili M (2009). Titanium dioxide. Journal of Nanotechnology. 8: 23-25.
Klančnik K, Drobne D, Valant J, Koce JD (2011) Use of a modified Allium test with nano TiO2. Ecotoxicology and environmental safety. 74 (1): 85-92.
Lamien-Meda A, Nell M, Lohwasser U, Börner A, Franz C, Novak J (2010) Investigation of antioxidant and rosmarinic acid variation in the sage collection of the gene bank in Gatersleben. Journal of Agricultural and Food Chemistry. 58(6): 3813-3819.
Larue C, Laurette J, Herlin-Boime N, Khodja H, Fayard B, Flank AM, Brisset F, Carriere M (2012) Accumulation, translocation and impact of TiO2 nanoparticles in wheat (Triticum aestivum spp.): influence of diameter and crystal phase. Science of the Total Environment. 431: 197-208.
Li Y, Horsman M, Wu N, Lan CQ, Dubois-Calero N (2008) Biofuels from microalgae. Biotechnology Progress. 24: 815-820.
Mahmoodzadeh H, Aghili R (2018) Effect on germination and early growth characteristics in wheat plants (Triticum aestivum L.) seeds exposed to TiO2 nanoparticles. Journal of Chemical Health Risks. 4 (1): 29-36.
Mandeh M, Omidi M, Rahaie M (2012) In vitro influences of TiO2 nanoparticles on barley (Hordeum vulgare L.) tissue culture. Biol Trace Elem Res. 150 (1-3): 376-380.
Marslin G, Sheeba CJ, Franklin G (2017) Nanoparticles Alter Secondary Metabolism in Plants via ROS Burst. Front Plant Sci. 19 (8): 832.
Martinez V, Mestre TC, Rubio F, Girones-Vilaplana A, Moreno DA, Mittler R, Rivero RM. (2016) Accumulation of flavonols over hydroxycinnamic acids favors oxidative damage protection under abiotic stress. Frontiers in plant science. 7: 1-17.
Mattiello A, Filippi A, Pošćić F, Musetti R, Salvatici MC, Giordano C, Vischi M, Bertolini A, Marchiol L (2015) Evidence of phytotoxicity and genotoxicity in Hordeum vulgare L. exposed to CeO2 and TiO2 nanoparticles. Frontiers in Plant Science. 6: 1-13.
Mehrabani B, Nazeri S, Piri KH (2013) Evaluation of total produced phenol in Chaei Koohi (Stachys lavandulifolia Vahi) callus culture and possibility of its enhancement using Elicitors. Agricultural Biotechnology Journal. 4 (2): 77-88.
Mittler R (2017) ROS are good. Trends in Plant Science. 22 (1): 11-19.
Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum. 15 (3): 473-497.
Nair R, Varghese SH, Nair BG, Maekawa T, Yoshida Y, Kumar DS (2010) Nanoparticulate material delivery to plants. Plant Science 179 (3): 154-163.
Nakano Y, Asada K (1981) Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant and Cell Physiology. 22 (5): 867-880.
Nour V, Trandafir I, Cosmulescu S (2014) Influence of preparing method on antioxidant activity and polyphenols content of green Walnuts comfiture.South-Western Journal of Horticulture, Biology and Environment. 5: 83-94.
Nourozi E, Hosseini B, Maleki R, Abdollahi Mandoulakani B (2019) Pharmaceutical important phenolic compounds overproduction and gene expression analysis in Dracocephalum kotschyi hairy roots elicited by SiO2 nanoparticles. Industrial Crops and Products. 133: 435-446.
Oloumi H, Soltaninejad R, Baghizadeh A (2015) The comparative effects of nano and bulk size particles of CuO and ZnO on glycyrrhizin and phenolic compounds contents in Glycyrrhiza glabra L. seedlings. Indian Journal of Plant Physiology. 20 (2): 157-161.
Pais I (1983) The biological importance of titanium. Journal of Plant Nutrition. 6: 3-131.
Palmqvist NGM, Bejai S, Meijer J, Seisenbaeva GA, Kessler VG (2015) Nano titania aided clustering and adhesion of beneficial bacteria to plant roots to enhance crop growth and stress management. Scientific Reports. 5: 1-12.
Schiavo S, Oliviero M, Miglietta M, Rametta G, Manzo S (2016) Genotoxic and cytotoxic effects of ZnO nanoparticles for Dunaliella tertiolecta and comparison with SiO2 and TiO2 effects at population growth inhibition levels. Science of the Total Environment. 550: 619-627.
Schrot J, Weng A, Melzing MF (2015) Ribosome-inactivating and relatead proteins. Toxins. 7 (5): 1556-615.
Sengul M, Ercisli S, Yildiz H, Gungor N, Kavaz A, Çetin B (2010) Antioxidant, antimicrobial activity and total phenolic content within the aerial parts of Artemisia absinthum, Artemisia santonicum and Saponaria officinalis. Iranian Journal of Pharmaceutical Research. 49-55.
Servin AD, Morales MI, Castillo-Michel H, Hernandez-Viezcas JA, Munoz B, Zhao L, Gardea-Torresdey JL (2013) Synchrotron verification of TiO2 accumulation in cucumber fruit: a possible pathway of TiO2 nanoparticle transfer from soil into the food chain.Environmental Science and Technology. 47 (20): 11592-11598.
Shallan MA, Hassan HM, Namich AA, Ibrahim AA (2016) Biochemical and physiological effects of TiO2 and SiO2 nanoparticles on cotton plant under drought stress. Research Journal of Pharmaceutical Biological and Chemical Sciences. 7 (4): 1540-1551.
Shin Y, Liu RH, Nock JF, Holliday D, Watkins CB (2007). Temperature and relative humidity effects on quality, total ascorbic acid, phenolics and flavonoid concentrations, and antioxidant activity of strawberry. Postharvest Biology and Technology. 45: 349-357.
Singh RS, Chattopadhyay T, Thakur D, Kumar N, Kumar T, Singh PK (2018) Hairy root culture for in vitro production of secondary metabolites: A promising biotechnological approach. In Kumar N. (eds). Biotechnological Approaches for Medicinal and Aromatic Plants.  Springer, Singapore. pp. 235-250.
Slinkard K, Singleton VL, (1977) Total phenol analysis: automation and comparison with manual methods. American Journal of Enology and Viticulture. 28 (1): 49-55.
Soares AMDS, Souza TFD, Jacinto T, Machado OLT (2010) Effect of methyl jasmonate on antioxidative enzyme activities and on the contents of ROS and H2O2 in Ricinus communis leaves.Brazilian Journal of Plant Physiology. 22(3): 151-158.
Srivastava S, Srivastava AK (2007) Hairy root culture for mass-production of high-value secondary metabolites. Critical Reviews in Biotechnology. 27 (1): 29-43.
Stripe F, Battelli M (2006) Ribosome-inactivating protens: progress and problems. Cellular and Molecular Life Sciences. 63 (16): 1850-66.
Sudhakar C, Lakshmi A, Giridarakumar S (2001) Changes in the antioxidant enzyme efficacy in two high yielding genotypes of mulberry (Morus alba L.) under NaCl salinity. Plant Science. 161 (3): 613-619.
Suriyaprabha R, Karunakaran G, Yuvakkumar R, Prabu P, Rajendran V, Kannan N (2012) Growth and physiological responses of maize (Zea mays L.) to porous silica nanoparticles in soil. Journal of Nanoparticle Research. 14 (12): 1294-1296.
Thiruvengadam M, Praveen N, John KM, Yang YS, Kim SH, Chung, I. M (2014) Establishment of Momordica charantia hairy root cultures for the production of phenolic compounds and determination of their biological activities. Plant Cell, Tissue and Organ Culture. 118 (3): 545-557.
Yang HG, Sun CH, Qiao SZ, Zou J, Liu G, Smith SC, Cheng HM, Lu GQ (2008) Anatase TiO2 single crystals with a large percentage of reactive facets. Nature. 453 (7195): 638-641.
Yousefi K, Riahi Madvar A (2016) Effect of flavone synthase gene expression and elicitor silver and copper on some biochemical parameters in seedlings of native Iranian cumin (Cuminum cyminum L). Journal of Plant (Iranian Journal of Biology). 28: 210-223.
Yousefi K, Riahi MA, Baghizadeh A (2015) Investigation of the effects of Ag and Cu elicitors on flavone synthase 1 gene expression and some biochemical parameters on Cuminum cyminum L. endemic to Iran. Journal of Plant Researches. 28 (1): 210-223.
Yuan SJ, Chen JJ, Lin ZQ, Li WW, Sheng GP, Yu HQ (2013) Nitrate formation from atmospheric nitrogen and oxygen photocatalysed by nano-sized titanium dioxide. Nature Communications. 4: 1-7.
Zhang L, Kai GY, Lu BB, Zhang HM, Tang KX, Jiang JH, Chen WS (2005) Metabolic engineering of tropane alkaloid biosynthesis in plants. J Integr Plant Biol. 47: 136-143.
Zhang Y, Yan X, Guo H, Zhao F, Huang L (2018) A novel protein elicitor BAR11 from Saccharothrix yanglingensis Hhs. 015 improves plant resistance to pathogens and interacts with catalases as targets. Frontiers in Microbiology. 9: 700-716.
Zhang Z, Liao L, Moore J, Wu T, Wang Z (2009) Antioxidant phenolic compounds from walnut kernels (Juglans regia L.). Food Chem. 113 (1): 160-165.
Žugić A, Đorđević S, Arsić I, Marković G, Živković J, Jovanović S, Tadić V (2014) Antioxidant activity and phenolic compounds in 10 selected herbs from Vrujci Spa, Serbia. Industrial Crops and Products. 52: 519-527.