Nano Biotechnology
Mohammad Ali Ebrahimi; Nassrin Qavami; Mahdi Dadmehr; Hadi Kalantari; Javad Shahghaghi; Ardeshir Qaderi; Nassim Zarinpanjeh
Abstract
Agriculture is facing many problems and dilemmas on the way to achieving the optimal performance of its products in terms of quantity and quality, due to the presence of various biotic and abiotic stresses such as pathogens, pests, weeds, inappropriate temperature and humidity, and many other factors. ...
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Agriculture is facing many problems and dilemmas on the way to achieving the optimal performance of its products in terms of quantity and quality, due to the presence of various biotic and abiotic stresses such as pathogens, pests, weeds, inappropriate temperature and humidity, and many other factors. It is predicted that by 2050, the world's population will reach about 9.6 billion people, in this way, agricultural production should increase between 70 and 100 percent in order to fulfill the responsibility of providing human food. Factors such as the shrinking of arable land, lack of water resources, climate change, and the reduction of the effectiveness of agricultural chemical inputs have intensified the problems caused by biotic and abiotic stresses for all types of crops. In this way, obtaining modern technologies and new findings to protect plants against stresses and improve the efficiency of using chemical inputs with the aim of ensuring food security in a healthy and sustainable manner is absolutely vital. Nano-biotechnology, which includes using nanostructures (substances smaller than one hundred nanometers) in biological applications, is a promising tool for realizing sustainable agriculture, which is a crucial factor in meeting the growing need for food in the world. In this paper, the general role of nanotechnology in the agricultural industry is reviewed as nano fertilizers, nano pesticides, nano growth regulators, nano water and soil remediators, etc.
Nano Biotechnology
Mansureh Rahmatyian; Mehdi Dadmehr; Mehdi Dadmehr
Abstract
Nanoparticles are one of the most applicable materials in, science such as biotechnology, and medicine due to their distinguished characters. The previous studies indicated that over the half of total production of nanoparticles is dedicated to the conventional and bio inspired synthesis of silver nanoparticles. ...
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Nanoparticles are one of the most applicable materials in, science such as biotechnology, and medicine due to their distinguished characters. The previous studies indicated that over the half of total production of nanoparticles is dedicated to the conventional and bio inspired synthesis of silver nanoparticles. These nanoparticles have antimicrobial characteristics and widespread applications in medicine and industry. Conventional chemical methods for synthesis of silver nanoparticles usually use the reducing agents that they are not compatible to environment and sometimes toxic and the resulting nanoparticles cannot be involved in biological applications. So, green synthesis of nanoparticles is the process that based on green chemistry for the synthesis of bio-nanoparticle is very important. In this study, eucalyptus (Eucalyptus obliqua) and mint (Mentha spicatae) extracts of medicinal plants for biological production of silver nanoparticles was used as a reducing agent. By adding the extract to the solution of silver nitrate at different temperatures and incubation times silver nanoparticles were produced. Changing the color from pale yellow to dark brown was showed the production of silver nanoparticles. There is also the strong peak absorption (SPR) at wavelengths between 420 and 460 with using spectroscopy UV/Vis which indicate the production of silver nanoparticles. The size of the nanoparticles was determined by electron microscopy analysis (SEM and TEM). The average sizes of nanoparticles in both plants were between 10 and 20 nm. The produced nanoparticles by the mint plant at the same condition was more stable than eucalyptus that confirmed that mint has more ability to produce silver nanoparticles.
Nano Biotechnology
Mehdi Dadmehr; Seyed Morteza Hosseini; Behnaz Korouzhdehi
Volume 5, Issue 11 , December 2015, , Pages 29-36
Abstract
Detection of foreign genes in genetically modified (GM) plants is very important for proving the effectiveness of genetic engineering procedure and determination of GM plants. In the present study a very sensitive and convenient fluorescence nanobiosensor for rapid detection of GM crops based on Fe3O4/Au ...
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Detection of foreign genes in genetically modified (GM) plants is very important for proving the effectiveness of genetic engineering procedure and determination of GM plants. In the present study a very sensitive and convenient fluorescence nanobiosensor for rapid detection of GM crops based on Fe3O4/Au core/shell nanoparticles was developed. Specific site of 35S CaMV, a well studied gene promoter in plant genetic engineering was used as the DNA sequence target. The core/shell Fe3O4/Au magnetic nanoparticles were synthesized to utilize their magnetic properties and improve their DNA functionalization. Fe@Au nanoparticles were functionalized by bounding of single stranded DNA (ssDNA) probe through sulfhydryl group at 5ʹ phosphate end. Then complementary target ssDNA were hybridized with immobilized ssDNA probe. Methylene blue was selected as a fluorescence probe. It was shown that methylene blue had significant interaction with hybridized DNA. Upon the addition of the target ssDNA, fluorescence intensity decreased in linear range by concentration of ssDNA from 3×10-7 to 2.2×10-9 M with detection limit of 1.2×10-10 M. The nanobiosensor enabled us to detect the transgenic crops through convenient and reliable simple method.
