Crop Biotechnology

In collaboration with Payame Noor University and Iranian Biotechnology Society

Current Issue

By Issue

By Author

By Subject

Author Index

Keyword Index

About Journal

Aims and Scope

Editorial Board

Publication Ethics

Indexing and Abstracting

Related Links

FAQ

Peer Review Process

Journal Metrics

News

Comparison of the effect of Azospirillum lipoferum, A. brasilense and Bacillus subtilis bacteria on vegetative and physiological of Lactuca sativa L.‎‏ indicators in order to induce plant growth and biological control of Pythium ultimum

Document Type : Research Paper

Authors

1 Department of Biology,, Faculty of Science, Payame Noor University, Tehran,, Iran

2 Department of Plant Pathology, Nuclear Agriculture Research School, Nuclear Science and Technology Research Institute (NSTRI), Atomic Energy Organization of Iran (AEOI), Alborz, Iran.

3 Department of Biotechnology, Payame Noor University, Tehran, Iran. & Department of Systems Biology, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), Iran.

4 Department of Biology, Faculty of Science, Payam e noor University, Tehran, Iran

10.30473/cb.2024.70671.1960

Abstract

Growth-promoting bacteria or plant probiotics are an effective part of biocompatible and sustainable agriculture. In this study, changes in some vegetatative i and plant physiological indicators and the biocontrol of P. ultimum (damping-off disease agent) in Lettuce plants treated with two types of growth stimulating bacteria (A lipoferum and A. brasilense) and a biocontrol species (B. subtilis and two gamma irradiated mutant isolates 419 and 600) were investigated in greenhouse conditions in the form of a completely randomized design (CRD) with four replications. The results showed bacterial treatments inthe all growth and physiological indicators, were significantly different from the control at the 5% level. In the P. ultimum inoculated treatments, the damping-off and diseases severity(DI) were significantly reduced as a result of the treatment with B. subtilis mutants obtained from gamma ray irradiation, but Azospirillium strains were not effective in biocontrol. The comparison of physiological inddexs in infected plants showed that peroxidase, polyphenol oxidase enzymes and proline increased and malonaldehyde decreased as a result of treatment with B. subtilis mutants. From the total results of this research, it can be concluded that the induction of mutation with gamma ray due to the improvement of antagonistic potential of B. subtilis ,leaded to increase the biological control activities of B. subtilis mutants without adverse effects on the growth and physiology of lettuce, so that the efficiency of these mutants in the biocontrol of pseudo-fungus P. ultimum and induction of physiological indicators of resistance in the lettuce plant is higher than that of Azosprilium efficiency.

Keywords

Main Subjects

References
Afsharmanesh, H., Ahmadzadeh, M., Javan-Nikkhah, M., & Behboudi, K. (2014). Improvement in biocontrol activity of Bacillus subtilis UTB1 against Aspergillus flavus using gamma-irradiation. Crop Protection, 60, 83-92.‏ (in Persian). Altunkaya, A.,et al. (2011). Purification and characterization of polyphenol oxidase, peroxidase and lipoxygenase from freshly cut lettuce (L. sativa). Food Technology and Biotechnology, 49(2), 249-256. Amaradasa, B. S., Mei, C., He, Y., Chretien, R. L., Doss, M., Durham, T., & Lowman, S. (2024). Biocontrol potential of endophytic Pseudomonas strain IALR1619 against two Pythium species in cucumber and hydroponic lettuce. Plos one, 19(2), e0298514. Constantinescu, F., Tomescu, A., Şesan, T. E., & Ştirbu, P. M. (2010, July). Biocontrol of Soil Borne Fungi in Tomato Crop by Using Beneficial Bacillus subtilis Strains. In III International Symposium on Tomato Diseases 914, 381-384.‏ Fatouros, G., Gkizi, D., Fragkogeorgi, G. A., Paplomatas, E. J., & Tjamos, S. E. (2018). Biological control of Pythium, Rhizoctonia and Sclerotinia in lettuce: association of the plant protective activity of the bacterium Paenibacillus alvei K165 with the induction of systemic resistance. Plant pathology, 67(2), 418-425.‏ Li DeQuan, L. D., Nie FengYa, N. F., Wei LiHui, W. L., Wei BenQiang, W. B., & Chen ZhiYi, C. Z. (2007). Screening of high-yielding biocontrol bacterium Bs-916 mutant by ion implantation.‏ Applied microbiology and biotechnology, 75, 1401-1408. Mangmang, J. S., Deaker, R., & Rogers, G. (2015). Optimal plant growth-promoting concentration of Azospirillum brasilense inoculated to cucumber, lettuce and tomato seeds varies between bacterial strains. Israel Journal of Plant Sciences, 62(3), 145-152. Mohamady, A., Abbas Akhavan Sepahi, A., & Hosseini Doust, S. R. (2017). Biological control of Pythium ultimum and Fusarium solani by indigenous strains Bacillus subtilis. Journal of Microbial Biology, 6(21), 1-14. (in Persian). Rezalou, Z., Shahbazi, S., Alilou, A. A., & Ghorbani, A. (2023). Biostimulant impact of Trichoderma species on physiological characteristics of beans. Iranian Journal of Genetics & Plant Breeding (IJGPB), 12(2). (in Persian). Rezalu, Z.,et al. (2022). Investigating the effect of growth-promoting bacteria in stimulating germination and improving the growth components of seeds. Iran seed science and technology.(in Persian). Romero, D., Vlamakis, H., Losick, R., & Kolter, R. (2011). An accessory protein required for anchoring and assembly of amyloid fibres in B. subtilis biofilms. Molecular microbiology, 80(5), 1155-1168. Rostaminia, M.,et al. (2021). Effect of three commercial bio-fertilizers prepared with Pseudomonas on yield and morphophysiological traits of lettuce (Lactuca sativa L.).‏ Iran Agricultural Research 39(2) 99-107.(in Persian). Sarti, G. C., Galelli, M. E., Arreghini, S., Cristóbal-Miguez, J. A. E., Curá, J. A., & Paz-González, A. (2023). Inoculation with Biofilm of Bacillus subtilis Promotes the Growth of Lactuca sativa. Sustainability, 15(21), 15406. Utkhede, R. S., Lévesque, C. A., & Dinh, D. (2000). Pythium aphanidermatum root rot in hydroponically grown lettuce and the effect of chemical and biological agents on its control. Canadian Journal of Plant Pathology, 22(2), 138-144. Yasari, E., & Patwardhan, A. M. (2007). Effects of (Azotobacter and Azosprillium) inoculants and chemical fertilizers on growth and productivity of canola (Brassica napus L.). Asian Journal of Plant Sciences.
Crop Biotechnology
Volume 13, Issue 46 - Serial Number 46
June 2024
Pages 91-106
Files
Share
How to cite
Statistics