Optimization of androgenesis in cucumber through anther culture by applying electric shock, centrifugation, and magnetic field treatments on anthers and induced calluses

Shafaee, Zahra; Abdollahi, Mohammad Reza; Moosavi, Sayyed Saeed; Sarikhani, Hassan

doi:10.30473/cb.2025.72510.1984

Optimization of androgenesis in cucumber through anther culture by applying electric shock, centrifugation, and magnetic field treatments on anthers and induced calluses

Articles in Press

Document Type : Research Paper

Authors

¹ Plant Production and Genetic Department, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran.

² Department of Horticulture Science, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran.

10.30473/cb.2025.72510.1984

Abstract

The induction of androgenesis in cucumber plays a significant role in accelerating breeding programs and improving desirable traits by producing doubled haploid plants. In the present study, the effects of different genotypes, different heat pretreatments, different levels of electric shock, different centrifugation speeds and different magnetic treatments on the androgenesis induction in cucumber anther cultures were investigated in 5 separate experiments based on completely randomized design with three replications. The results showed that the highest percentage of androgenesis induction and embryogenesis was related to the beta-alpha genotype. The effect of applying heat pretreatment in liquid culture medium on embryogenesis and plant regeneration was significant, so that the control treatment in liquid culture medium with an average of 4.36 plants per anther and then the 30°C treatment in liquid culture medium with an average of 1.4 plants per anther were the best treatments. The liquid medium was also very effective for embryogenesis, and a large number of plants were regenerated in this method. 100 V electric shock treatment showed the highest percentage of callus formation with 90% and the highest average number of embryos per anther with 0.16 embryos. Centrifuge treatment (150 g) produced the highest percentage of callus formation (73.33%) and the highest average number of embryos per anther (0.2). Magnetic water passage treatment with an average of 0.2 embryo per anther was more suitable than other magnetic treatments. The results of this research can be used in the production of pure cucumber lines in order to produce hybrid seeds.

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