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آنالیز بیوانفورماتیکی فاکتور رونویسی MADS-box در گیاه Brachypodium distachyon

نوع مقاله : علمی پژوهشی

نویسندگان

1 دانشجوی دکتری، گروه زیست‌فناوری گیاهی و بیوتکنولوژی، دانشکده علوم و فناوری‌زیستی، دانشگاه شهید بهشتی، تهران، ایران

2 استاد گروه زیست‌فناوری گیاهی و بیوتکنولوژی، دانشکده علوم و فناوری‌زیستی، دانشگاه شهید بهشتی، تهران، ایران

چکیده

آغاز گلدهی فاکتور مهمی است که عملکرد گیاه را تحت تأثیر قرار می‌دهد. عوامل محیطی تأثیر معنی‌داری بر مرحله گلدهی می‌گذارند. آنالیز بیوانفورماتیک فاکتور رونویسی MADS-box که به عنوان اجزای مهم در تشکیل گلدهی انجام گرفت. برکپودیوم گیاه مدل جدیدی است که برای درک بهتر مکانیسم‌های ژنتیکی، سلولی و بیولوژی مولکولی گیاهان مورد استفاده قرار می‌گیرد. در این مطالعه 43 توالی ژن‌های MADS-box برکپودیوم با استفاده از روابط فیلوژنی، موتیف‌های محافظت‌شده، نقشه کروموزومی، آنالیز جایگاه اتصال فاکتور رونویسی و ترکیبات آمینواسیدهای آنالیز شدند. هدف از این مطالعه شناخت بهتر مکانیسم‌های مولکولی مرتبط با گلدهی می‌باشد. در این مطالعه، نتایج نشان داد که ژن‌هایMADS-box بر روی تمامی کروموزوم‌های برکپودیوم پراکنده هستند، درحالی‌که کلاسترهای ژنی بر روی تمامی کروموزم‌ها به جز کروموزم شماره پنج قرار داشتند. آنالیز ترکیبات آمینواسیدی نشان داد که لوسین، سرین و گلوتامات بالاترین مقدار و پایین‌ترین میزان مربوط به تریپتوفان بود که باعث القای گلدهی می‌شود. براساس آنالیز فیلوژنی ژن‌ها به 4 گروه تقسیم بندی شدند. تست تاجیما وجود انتخاب متعادل را در توالی MADS-box پیش‌بینی می‌کند و درنتیجه، پلی‌مورفیسم در توالی‌ها حفظ می‌شود. در نتیجه می‌توان گفت که تنوع کل در ژن‌های MADS-box بالا بوده‌است. در مجموع، نتایج ما اطلاعات مفیدی برای بررسی ژن‌های درگیر در پاسخ به گلدهی فراهم نموده و شناخت مکانیسم مولکولی و روابط بین ژنی در مسیر گلدهی را تسهیل ساخته‌است.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Bioinformatics analysis of MADS-box in Brachypodium distachyon

نویسندگان [English]

  • Zahra Hajibarat 1
  • Abbas Saidi 2
  • Zohreh Hajibarat 1

1 Ph.D. Candidate, Department of Plant Biology & Biotechnology, Faculty of Bioscience and Biotechnology, Shahid Beheshti University, Tehran, Iran.

2 Professor, Department of Plant Biology & Biotechnology, Faculty of Bioscience and Biotechnology, Shahid Beheshti University, Tehran, Iran

چکیده [English]

Flower initiation is an important factor influencing plant yield. Environmental factors significantly affect flowering initiation. Bioinformatic analysis was performed on MADS-box transcription factors which are considered as important components in the flower formation. Brachpodium is a new experimental model which used to understand the genetic, cellular mechanism and molecular biology of plants. In this study, 43 sequences of Brachypodium MADS-box genes were analyzed using phylogeny relationships, conserved motifs, chromosomal location, detection of transcription factor binding sites, and amino acid composition. The aim of this study was to better identify molecular mechanisms related to flowering. In this study, results showed that MADS-box genes distribute on all Brachypodium chromosomes, while gene clusters were located on all chromosomes except chromosome five. Analysis of the amino acid composition revealed that lucine, serine, and glutamate, with the highest amount, and tryptophan, with the least amount, elicit appreciable flowering. Based on the phylogeny analysis the genes were divided to four clusters. Tajima test indicated the presence of balancing selection in MADS-box sequences and as a result polymorphism is conserved in the sequences. Thus, the total diversity in MADS-box genes were high. Overall, our results provided useful information for the survey of flowering response genes, thereby detection of molecular mechanism and intergenic relationships facilitate flowering pathway.

کلیدواژه‌ها [English]

  • Phylogeny
  • MADS-box
  • Transcription factor
  • Flowering
  • Polymorphism
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فصلنامه علمی زیست فناوری گیاهان زراعی
دوره 8، شماره 23
آبان 1397
صفحه 1-15
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هم رسانی
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