Evaluation of enzymatic and non-enzymatic defense mechanism in response to Charcoal Rot disease during growth stage in soybean

Navabpour, Saeid; Mirkarimi, Shahab; Mazandarani, Abolfazl

Evaluation of enzymatic and non-enzymatic defense mechanism in response to Charcoal Rot disease during growth stage in soybean

Document Type : Research Paper

Authors

¹ Assistant professor of Plant Breeding, Department of Plant Breeding and Biotechnology, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran,

² Graduated student of Plant Breeding, Islamic Azad University Science and Research Branch

³ Graduated student of Plant Breeding, Isfahan University of Technology, Isfahan, Iran

Abstract

Charcoal Rot is one of the most important fungal diseases in soybean particularly in northern region of Iran. This disease caused some damages to yield quality and quantity (up to 20%) especially in dried and worm seasons. Today there is not many researches in regards to enzymatic and non-enzymatic study in response to disease. In this study super oxide and hydrogen peroxide radicals have measured, also, super oxide dismutase, and catalase enzymes, as well as ascorbic acid changes, alphatochopheroll, carotenoid and TBARM (cellular oxidative levels) in soybean genotypes during growth stages (flowering, packing pod and grain filling) at two environmental conditions (control and disease). The result showed superoxide and hydrogen peroxide radicals content have increasing during growth stages, as the most amount accorded in final stage of sampling (grain filling). Katul cultivar and DPX × For a hybrid had more tolerance to charcoal rot disease due to antioxidant defense systems.

Keywords