Proteomics
Mohammad Reza Azimi; Ghasem Hosseini Salekdeh
Volume 4, Issue 7 , December 2015, , Pages 1-13
Abstract
Water shortage is one of the most important environmental factors in limiting plant production worldwide. Molecular breeding may help to develop drought tolerant plants. Proteomics approach can help in comprehensive analysis of stress responsive genes and identification of drought signaling pathways. ...
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Water shortage is one of the most important environmental factors in limiting plant production worldwide. Molecular breeding may help to develop drought tolerant plants. Proteomics approach can help in comprehensive analysis of stress responsive genes and identification of drought signaling pathways. These drought tolerance candidate genes or their regulatory genes may be further analyzed for their possible implication in increase plant tolerance to drought stress. In this studied we analyzed the changes in proteome of wheat flag leaf in response to drought. We compared a drought tolerant with drought susceptible genotypes under normal and stress well-watered and stress conditions. Stressed plants were exposed to 50% field capacity before anthesis. Out of 900 proteins analyzed across two dimensional gels, 57 protein spots showed significant differences in response to stress. Of these, 42 protein spots could be identified using mass spectrometry analysis. Differentially expressed proteins and enzymes could be grouped in different functional groups. Our results showed that tolerant genotype may use various mechanisms particularly the up-regulation of genes involved in oxidative stress defense in flag leaf. This may help the tolerant genotypes to better remove reactive oxygen species generate by stress in flag leaf and maintain its physiological and photosynthetic activities.
Biotic and Abiotic stress
Sepideh Torabi; Ghasem Hosseini Salekdeh
Volume 3, Issue 4 , September 2013, , Pages 1-14
Abstract
R Rice is one of most important crops feeding about half of the world population. Phosphorous is one of limiting factors of rice growth and its deficiency substantially decreases rice yield. In this study, we aimed to identify biochemical mechanisms of rice adaptation to phosphorous deficiency in leaf ...
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R Rice is one of most important crops feeding about half of the world population. Phosphorous is one of limiting factors of rice growth and its deficiency substantially decreases rice yield. In this study, we aimed to identify biochemical mechanisms of rice adaptation to phosphorous deficiency in leaf and root. We compared the metabolites in parental lines, Nipponbare, and its near isogenic line (NIL6-4) containing a major QTL for phosphorous uptake (PUP1) on chromosome 12. Phosphorous deficiency resulted in significant decrease in number of tillers and fresh weight in both parental line and its near isogenic line, however, this reduction was more pronounced in the parental line. Analysis of the level of several soluble sugars and activity of enzymes involved in energy production revealed that phosphorous deficiency could lead to some changes in glycolysis pathway in rice near isogenic line so that the plant could cope with the ATP shortage. It is most likely that enhancement of this biochemical pathways may increase rice adaptation to phosphorous deficiency and consequently increase plant production with less phosphorus fertilizer required.
Biotic and Abiotic stress
Behnam Bakhshi; Mohmmad Reza Bihamta; Ghasem Hosseini Salekdeh; Masoud Tohidfar
Volume 3, Issue 5 , February 2013, , Pages 93-102
Abstract
Drought stress is one of the most important abiotic stresses that deteriorates rice agriculture of Iran. One of the best ways to establish drought stress tolerance in plants is miRNA mediated post transcriptional gene regulation. MiRNAs are small 19-24 nt regulatory RNAs and play important role in regulating ...
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Drought stress is one of the most important abiotic stresses that deteriorates rice agriculture of Iran. One of the best ways to establish drought stress tolerance in plants is miRNA mediated post transcriptional gene regulation. MiRNAs are small 19-24 nt regulatory RNAs and play important role in regulating plant gene expression in biotic and abiotic stress. In this study, we selected five miRNAs for promoter analysis and evaluation of differential expression of them under drought stress in roots. Three of them including miR162, miR169 and miR172 are conserved in many plants and the others including miR1425 and miR1880 are rice specific miRNAs. In addition, upstream screening of MIRNA genes showed that upstream region of some MIRNA genes like MIR172 are enriched with important regulatory elements like DRE and ABRE. Quantitative Realtime-PCR was used in this study for analyzing differential expression of evaluated miRNAs. Studying the differential expression of miRNAs in roots under drought condition showed that miR169 was up-regulated but conversely, miR172 was down-regulated. The rest of miRNAs in our study did not show significant differential expression under drought stress. It can be concluded that NF-YA and AP2 as the most important target genes for miR169 and miR172 respectively can play critical roles in response to drought stress. .
A Hashemi; GH Nematzadeh; GH Hosseini Salekde; A Hosseini; M.R Hajirezaei
Volume 2, Issue 2 , September 2012, , Pages 1-14
Abstract
Two rice lines, IR29 and FL478, which differed in salinity tolerance, were investigated for physiological and metabolic responses
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Two rice lines, IR29 and FL478, which differed in salinity tolerance, were investigated for physiological and metabolic responses
Proteomics
Ghasem Hosseini Salkade; Davood Nasr Abadi
Volume 1, Issue 1 , March 2012, , Pages 1-11
Abstract
Rice is an excellent model cereal for molecular biology and genetics research. Salinity is a major factor limiting rice production world wide. The analysis of stress-responsiveness in plants is an important route to the discovery of genes conferring stress tolerance and their use in breeding programs. ...
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Rice is an excellent model cereal for molecular biology and genetics research. Salinity is a major factor limiting rice production world wide. The analysis of stress-responsiveness in plants is an important route to the discovery of genes conferring stress tolerance and their use in breeding programs. To further understand the mechanism of plant response to salinity we employed a proteomic approach to profile the protein changes of rice 3th leaf and root under salt stress. Plants were grown in Yoshida nutrient solution and salt stress imposed after 25 days. Plants were treated by 100¬mM NaCl for 10. After that 3th leaves and total root were collected from control and salt stressed plants. The Na+ and K+ content of leaves/roots and several yield components changed significantly in response to short-term salt stress and their proteome patterns were analyzed using 2-DE in triplicates. The expression pattern of proteins significantly changed in all leaves/roots in response to stress. More than 488 and 345 protein were detected repeatedly in root and leaf 2Dgels respectively by software package. 107 proteins in root and 86 proteins in leaf of two genotypes showed significant response to stress. 3 protein in leaf gels and 2 protein in root gels were selected and identified by ESI-Q-TOF. The most important were Ferritin, Rubisco activase and ascorbat¬peroxidase in leaf and Peroxidase and Ascorbat¬peroxidase in root. All of them were enzyme and involved in detoxification and removal of reactive oxygen species (peroxidase, ascorbat¬peroxidase) Iron homeostasis (ferritin) or activation of other enzymes (rubisco¬activase).
