Proteomics
Mohammad Reza Azimi; Ghasem Hosseini Salekdeh
Volume 4, Issue 7 , December 2015, , Pages 1-13
Abstract
Water shortage is one of the most important environmental factors in limiting plant production worldwide. Molecular breeding may help to develop drought tolerant plants. Proteomics approach can help in comprehensive analysis of stress responsive genes and identification of drought signaling pathways. ...
Read More
Water shortage is one of the most important environmental factors in limiting plant production worldwide. Molecular breeding may help to develop drought tolerant plants. Proteomics approach can help in comprehensive analysis of stress responsive genes and identification of drought signaling pathways. These drought tolerance candidate genes or their regulatory genes may be further analyzed for their possible implication in increase plant tolerance to drought stress. In this studied we analyzed the changes in proteome of wheat flag leaf in response to drought. We compared a drought tolerant with drought susceptible genotypes under normal and stress well-watered and stress conditions. Stressed plants were exposed to 50% field capacity before anthesis. Out of 900 proteins analyzed across two dimensional gels, 57 protein spots showed significant differences in response to stress. Of these, 42 protein spots could be identified using mass spectrometry analysis. Differentially expressed proteins and enzymes could be grouped in different functional groups. Our results showed that tolerant genotype may use various mechanisms particularly the up-regulation of genes involved in oxidative stress defense in flag leaf. This may help the tolerant genotypes to better remove reactive oxygen species generate by stress in flag leaf and maintain its physiological and photosynthetic activities.
Proteomics
Ghasem Hosseini Salkade; Davood Nasr Abadi
Volume 1, Issue 1 , March 2012, , Pages 1-11
Abstract
Rice is an excellent model cereal for molecular biology and genetics research. Salinity is a major factor limiting rice production world wide. The analysis of stress-responsiveness in plants is an important route to the discovery of genes conferring stress tolerance and their use in breeding programs. ...
Read More
Rice is an excellent model cereal for molecular biology and genetics research. Salinity is a major factor limiting rice production world wide. The analysis of stress-responsiveness in plants is an important route to the discovery of genes conferring stress tolerance and their use in breeding programs. To further understand the mechanism of plant response to salinity we employed a proteomic approach to profile the protein changes of rice 3th leaf and root under salt stress. Plants were grown in Yoshida nutrient solution and salt stress imposed after 25 days. Plants were treated by 100¬mM NaCl for 10. After that 3th leaves and total root were collected from control and salt stressed plants. The Na+ and K+ content of leaves/roots and several yield components changed significantly in response to short-term salt stress and their proteome patterns were analyzed using 2-DE in triplicates. The expression pattern of proteins significantly changed in all leaves/roots in response to stress. More than 488 and 345 protein were detected repeatedly in root and leaf 2Dgels respectively by software package. 107 proteins in root and 86 proteins in leaf of two genotypes showed significant response to stress. 3 protein in leaf gels and 2 protein in root gels were selected and identified by ESI-Q-TOF. The most important were Ferritin, Rubisco activase and ascorbat¬peroxidase in leaf and Peroxidase and Ascorbat¬peroxidase in root. All of them were enzyme and involved in detoxification and removal of reactive oxygen species (peroxidase, ascorbat¬peroxidase) Iron homeostasis (ferritin) or activation of other enzymes (rubisco¬activase).
