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Detection and differentiation of two complications of disorder and lack of podding of soybeans in the fields of Golestan and Mazandaran provinces

Document Type : Research Paper

Authors

1 PhD student in Plant Pathology, Gorgan University of Agricultural Sciences and Natural Resources, Golestan

2 Associate Professor of Plant Protection, Plant protection department, Gorgan University of Agricultural Sciences and Natural Resources, Golestan

3 Assistant Professor of Plant protection, Gorgan University of Agricultural Sciences and Natural Resources, Golestan,

4 Associate Professor of Plant breeding and biotechnology department, Gorgan University of Agricultural Sciences and Natural Resources, Golestan

Abstract

In order to detection and differentiation of two complications of disorder and lack of podding in soybeans, while visiting 185 soybean fields in Golestan and Mazandaran provinces, only from the summer cultivation of 17 fields from five different places in Golestan province, plants with signs of disorder and the lack of acute podding were identified and 17 samples were selected from each field and sampling was done from the leaves or stems of the mentioned plants in order to extract RNA and DNA. Then, PCR was performed to detect nepovirus using the degenerate primer of nepovirus and to detect phytoplasma using a pair of general primers and a nested PCR test. The results of electrophoresis confirmed the amplification of the 1800 bp band in the general PCR, the 1250 bp fragment in the nested PCR related to phytoplasma, and also the amplification of the 640 bp band related to a nepovirus. Besides, no band of healthy plants was observed. at the same time, tissue grafting and mechanical inoculation were performed on GPX soybean benchmark plant using the mentioned samples and two types of symptoms appeared. From the sequencing of the disordered samples (production of a small number of seeds and small pods), Tomato ring spot virus strain ep31_63026 and from the sequencing of the samples with non-encapsulation (grassiness and no formation of pods and seeds), Aster yellows phytoplasma from the 16SrI-B group were identified, which confirmed the results of the reference plant. The phylogenetic analysis of sequencing results confirmed the presence of Phytoplasma and Nepovirus only in the summer culture of samples that had symptoms of disorder and lack of podding.

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References
Allington, W. B. (1946). Bud blight of soybean caused by the Tobacco ring spot virus. Phytopathology. 36(4), 319-322.ALLINGTON, W. B. Arocha, Y., Peralta, E. L., & Jones, P. (2023). Genetic characterization of phytoplasmas associated with yellow leaf syndrome (YLS) in Cuba. Phytopathology, CENSA, Apdo 10. Carretera. Bertin, S., Galletti, R., Paltrinieri, S., & Contaldo, N. (2018). Phytoplasma and virus diseases of soybean in Italy: state of the art and future perspectives. Journal of Plant Pathology, 100(3), 403-413. Cheng, R., Yan, R., Mei, R., Wang, Y., Niu, W., Ai, H., Qiao, S., Xu, M., Yu, W., Ye, W., Wang, Y., Tao, X., Zhou, X., & Xu, Y. (2023). Epidemiological evaluation and identification of the insect vector of 2 soybean stay-green associated virus. Biorxiv. https://doi.org/10.1101/2023.03.13.532384 Digiaro, M., Elbeaino, T., & Martelli, G. P. (2007). Development of degenerate and species-specific primers for the differential and simultaneous RT-PCR detection of grapevine-infecting nepoviruses of subgroups A, B and C. Journal of virological methods, 141(3): 34-40. Deng, S. J, & Hiruki, C. (1991). Amplification of 16S ribosomal-RNA genes from culturable and nonculturable mollicutes. J Microbiol Methods, 14(1), 53-61. Elmore, M. G., Groves, C. L, Hajimorad, M. R. et al. (2022). Detection and discovery of plant viruses in soybean by metagenomic sequencing. Virol J, 19, 149. https://doi.org/10.1186/s12985-022-01872-5. FAO. (2023). FAOSTAT: Production - Crops, 2023 data. Retrieved from http://www.fao.org/faostat/en/#data/QC. Farzadfar, Sh., Golnaragi, A. R., & Pourrahim, R. (2002). Plant Viruses of Iran. Saman Co. Tehran. Iran. 145p. Garison, A. S. (2004). Tobacco ring spot virus. http://www.soydiseases.uiuc.edu/diseases. Golnaraghi, A. R, Shahraeen, N., Purrahim, R., Farzadfar, Sh., & Ghasemi, A. (2002). First report of the natural occurrence of eight viruses affecting soybeans in Iran. New Disease Reports, 5 (1). Gundersen, D. E, & Lee, I-M. (1996). Ultrasensitive detection of phytoplasmas by nested-PCR assays using two universal primer pairs. Phytopathol Mediterr, 35, 144-151. Haudenshield, J. S, Hartman, G. L., & Kabbage, M. (2018). Management of Soybean Diseases. In J. R. Smith & H. W. Hunt (Eds.), Soybean: Improvement, Production, and Uses (pp. 445–486). American Society of Agronomy, Inc., Crop Science Society of America, Inc., Soil Science Society of America, Inc. https://doi.org/10.2134/agronmonogr63.c13. Heinrich, M., Botti, S., Caprara, L., Arthrofer,W., Strommer, S., & Hanzer, V. (2001). Improved detection method for fruit tree phytoplasmas. Plant Mol. Biol. Rep. Egyptian J. Virol, 11 (2), 35-45, 2014. 19, 169-179. Jović, J., Cvrković, T., Mitrović, M., Krnjajić, S., Petrović, A., Redinbaugh, M. G, Pratt, R.C, Hogenhout, S.A, & Tosevski, I. (2009). Stolbur phytoplasma transmission to maize by Reptalus panzeri and the disease cycle of maize redness in Serbia. Phytopathology, 99(9), 1053-1061. https://doi.org/10.1094/PHYTO-99-9-1053. Lee, I. M., Martini, M., Marcone, C., & Zhu, S. F. (2004). Classification of phytoplasma strains in the elm yellows group (16SrV) and proposition of ‘Candidatus Phytoplasma ulmi’ for the Phytoplasma. Int. J. Syst. Evol. Microbiol. Mar 54(Pt 2), 337-47 Lee, I. M., Hammond, R. W., Davis, R. E et al. (1993). Universal amplification and analysis of pathogen 16S rDNA for classification and identification of mycoplasma like organisms. Phytopathology, 83, 834-842. Li, R., Li, Y., Zhang, Y., Liu, X., & Wang, Q. (2019). Co-infection of soybean with Soybean mosaic virus and phytoplasma reduces yield and quality. Plant Disease, 103(4), 708-714. Jadhav Pravin, V., Mane, S. S, Nandanwar, R. S., Kale, P. B., Dudhare, M. S., & Moharil, M. P. (2015). Floral bud distortion in soybean and incidence in Central India. Egyptian Journal of Biology, 15, 59-65. Koenning, S. R, & Wrather, J. A. (2010). Suppression of Soybean Yield Potential in the Continental United States by Plant Diseases from 2006 to 2009. Plant Health Progress, 11(1), 1-9. https://doi.org/10.1094/PHP-2010-0106-01-RS. Kube, M., Mitrovic, J., Duduk, B., Rabus, R., & Seemuller, E. (2012). Current View on Phytoplasma Genomes and Encoded Metabolism. The Scientific World Journal, 1(1). Rahimian, H., Hamdollazadeh, A., & Motazari, M. (1996). Identification of viral agent associated with Soybean podding disorder. Iranian J of Plant Pathology, 32(1), 491-498. Sedano, R. E., & Morales, F. (2023). Aster yellow phytoplasma adapted to Phaseoulus vulgaris L. in ElSalvador, Colombia Central America. JOURNAL Virology, NCBI.Submitted (04-FEB-2002). Shameli, S., Shahraeen, N., & Safarnejad, M. R. (2022). Assessment of Yeild and Soybean Cultivars Response to Nepoviruses, and Possible Effect of Nepoviruses on Soybean Podding Disorder. Journal of Plant Protection, 29(4): 165-183. Sincklair, J. B., & Backman, P. A. (2014). Compendium of soybeam diseases. American Phytopathological Society, 147-149. Soufi, Z., & Komor, E. (2014). Latent infection of asymptomatic Hawaiian sugarcane cultivars with 16SrI and 16SrXI phytoplasmas. Journal of General Plant Pathology, 80, 255-263. Schneider, B., Seemu¨ller, E., Smart, C. D., et al. (1995). Phylogenetic classification of plant pathogenic mycoplasma like organisms or phytoplasmas. In: Razin S, Tully JG (eds) Molecular. Molecular and Diagnostic Procedures in Mycoplasmology, 1(2). Tu, J. C. (1986). Strains of Tobacco ring spot virus isolated from soybean (Glycine max) in southwestern Ontario (Canada). Canadian Journal of Plant Science, 66, 491-498. Malapi-Nelson, M., Wen, R. H., Ownley, B. H., & Hajimorad, M. R. (2009). Co-infection of Soybean with Soybean mosaic virus and Alfalfa mosaic virus Results in Disease Synergism and Alteration in Accumulation Level of Both Viruses. Plant disease, 93(12), 1259-1264. https://doi.org/10.1094/PDIS-93-12-1259 Weintraub, P., & Jones, P. (2009). PHYTOPLASMAS Genomes, Plant Hosts and Vectors, CAB International, United Kingdom.432p. Wu, Y., Huang, L., Zhang, J., & Chen, Y. (2020). RNA interference technology for controlling plant viruses and phytoplasmas. Frontiers in Microbiology, 11, 1168. Yu, S., & Zhu, A. N. (2023). Carica papaya Represents a New Host of 16SrI-B Subgroup Phytoplasma Associated with Yellow Symptoms in China. Plant Protection Tropical Center, NCBI. Ziems, A. D., Giesler, L. J., Graef, G. L., Redinbaugh, M. G., Vacha, J. L., Berry, S., Madden, L., & Dorrance, A. (2007). Response of Soybean Cultivars to Bean pod mottle virus Infection. Plant Disease, 91(6), 719-726.
Crop Biotechnology
Volume 12, Issue 41 - Serial Number 41
June 2023
Pages 49-60
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