Document Type : Research Paper
Authors
- aliakbar babajanpour bora 1
- Gholamali Ranjbar 2
- Seyyed Hamidreza Hashemi-petroudi 3
- Hamid Najafi Zarini 4
- Ghorbanali Nematzadeh 5
1 student
2 Department of Biotechnology, Sari Agricultural Sciences and Natural Resources University (SANRU), Sari, Iran. 3. Professor, Department of Genetic Engineering and Biology, Genetics and Agricultural Biotechnology Institute of Tabarestan (GABIT), Sari A
3 Department of Genetic Engineering and Biology, Genetics and Agricultural Biotechnology Institute of Tabarestan (GABIT), Sari Agricultural Sciences and Natural Resources University (SANRU)
4 Sari Agricultural Sciences and Natural Resources University (SANRU)
5 Sari Agricultural Sciences and Natural Resources University, University of the Philippines Los Banos
Abstract
Cyanobacteria improve soil fertility and organic product productivity by synthesizing growth-promoting substances, phosphate solubilization, and biological nitrogen fixation. We investigated the effect of eight various cyanobacterial strains on the germination-stage development of three different rice varieties namely Fajr, Roshan, and Tarem. The ammonium transporter (AMT) gene family was studied because ammonium is the most favored form of nitrogen that flooded rice can absorb. The majority of the experimental parameters were considerably impacted by cyanobacteria. In contrast to the qualitative cultivar Tarem Hashemi, germination characteristics were considerably enhanced when eight cyanobacteria strains were co-cultivated with two high-yielding cultivars, Roshan and Fajr. The amounts of nitrate and ammonium were highest in strain 7, with concentrations of 0.08 μg/ml and 0.010 μg/ml, respectively, out of the eight strains tested for nitrogen excretion. In silico analysis discovered 12 gene loci and 15 OsAMT isoforms in the rice genome. Examining the OsAMT gene family members in protein domain-specific databases revealed that all of the examined genes (excluding OsAMT3;4) include an ammonium-transporting protein domain. Three groups of OsAMTs were identified based on their gene structures and evolutionary relationships; each group shared common motif patterns and exon/intron order. The identification of active and inactive OsAMT genes in bioinformatic analysis could bring new insights into functional genomics studies of the rice ammonium transporter gene family, particularly in co-cultivation with cyanobacteria.
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