Microspore embryogenesis in different hybrids of bread wheat (Triticum aestivum L) and improvement of embryogenesis efficiency via 2,4-D stress pre-treatment

Tayefe-Afshari, Bahareh; Enayati Shariat Panahi, Mehran; Vahab-Zadeh, Mojtaba

Microspore embryogenesis in different hybrids of bread wheat (Triticum aestivum L) and improvement of embryogenesis efficiency via 2,4-D stress pre-treatment

Document Type : Research Paper

Authors

Researcher and head of Tisue culture and Gene transformation Dep.

Abstract

The objective of this study was to improve induction of embryogenesis in bread wheat microspores. In this study, some F1 hybrids i.e M85-6 × 90, M85-8 × 90, mv17 × shiroudi, mv17 × kavir and kavir × bam were used. Microspores were cultured in A2 medium containing different amounts of maltose (60¬ & ¬90 ¬g/lit) depending on the genotype used. Analysis of variance for embryogenesis and regenerable embryos showed highly significant difference between hybrids but there was no significant difference between media (A2-60¬ &¬ A2-90) and interaction effects. M85-6 × 90, mv17 × Shiroudi and mv17 × kavir produced the highest ratio of embryogenesis among the hybrids. In regeneration phase, mv17 × Shiroudi and M85-6 × 90 had the highest frequency of regenerable embryos. The effect of 2,4-D as a novel stress for induction of microspores embryogenesis in Falat -known as the most responsive wheat cultivar to microspore culture technology, was investigated. Microspores were subjected to 2,4-D at 3 concentrations including 15, 25, 35 mg/l for 30 minutes while microspores without any stress treatment were used as the control. The embryogenesis of microspores stressed with 2,4-D were better than control. The highest yield of embryogenesis was produced at 15 mg/l 2,4-D. The most regenerated embryos were obtained in 15 & 25 mg/l of 2,4-D. According to the results obtained, 2,4-D is introduced as a new stress for induction of embryogenesis in microspores of wheat.

Keywords