Evaluation of the effect of Iranian johenson grass mosaic virus infection on some morphological and phenological traits of twelve maize hybrids

Document Type : Research Paper

Authors

1 Ph.D. Student, Department of Plant Protection, Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.

2 Associate Professor, Department of Plant Protection, Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.

3 Associate Professor, Department of Plant Breeding and Biotechnology, Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.

Abstract

A B S T R A C T
Corn (Zea mays) belongs to Poaceae family. In recent years, the cultivated area and its uses have increased rapidly in most countries of the world. The Iranian johenson grass mosaic virus is one of the most important potyviruses of cereals, and the most effective way to combat it is to use resistant cultivars. In the present study, the response of 12 corn hybrids to this virus was investigated using a randomized complete block design. The extract of infected plants was inoculated mechanically on the leaves of corn hybrids at the four-leaf stage. Indirect ELISA test and RT-PCR test were performed using specific primers in order to ensure infection of the samples using IJMV specific antibody. One month after inoculation, notes were taken of phonological and morphological characteristics and measurements of the severity and percentage of infection showed significant differences between treatments. The results of the indirect ELISA test showed that the inoculated samples reacted positively with the virus. After performing the RT-PCR test, the amplification of a fragment of 327 base pairs in the inoculated samples indicated the presence of IJMV. By examining all traits, especially the severity and percentage of infection and yield, the hybrids were categorized. So that hybrids No. 3 and 4 (KLM78023/35-1-1-1-1× MO17 and K47/2-2-1-4-1-1-1 x K18) were more susceptible to IJMV virus, but hybrids No. 5 and 12 (KSC 705 and K47/2-2-1-2-2-1-1-1× K18 (KSC715) were relatively resistant or tolerant to the virus. Therefore, they are recommended for use in future corn breeding work.

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References

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