The effect of Azospirillum lipoferum, A. brasilense and Bacillus subtilis bacteria on biological control of Pythium ultimum

Document Type : Research Paper

Authors

1 Department of Biology, Faculty of Science, Payame Noor University, Tehran, Iran.

2 Department of Plant Pathology, Nuclear Agriculture Research School, Nuclear Science and Technology Research Institute (NSTRI), Atomic Energy Organization of Iran (AEOI), Alborz, Iran.

3 Department of Biotechnology, Payame Noor University, Tehran, Iran. & Systems Biology Research Department, ABRII, Agricultural Research, Education, and Extension Organization (AREEO), Karaj, Iran.

4 Department of Biology, Faculty of Science, Payam e noor University, Tehran, Iran

10.30473/cb.2024.70671.1960

Abstract

Growth-promoting bacteria or plant probiotics are an effective part of biocompatible and sustainable agriculture. In this study, changes in some vegetatative i and plant physiological indicators and the biocontrol of P. ultimum (damping-off disease agent) in Lettuce plants treated with two types of growth stimulating bacteria (A lipoferum and A. brasilense) and a biocontrol species (B. subtilis and two gamma irradiated mutant isolates 419 and 600) were investigated in greenhouse conditions in the form of a completely randomized design (CRD) with four replications. The results showed bacterial treatments inthe all growth and physiological indicators, were significantly different from the control at the 5% level. In the P. ultimum inoculated treatments, the damping-off and diseases severity(DI) were significantly reduced as a result of the treatment with B. subtilis mutants obtained from gamma ray irradiation, but Azospirillium strains were not effective in biocontrol. The comparison of physiological inddexs in infected plants showed that peroxidase, polyphenol oxidase enzymes and proline increased and malonaldehyde decreased as a result of treatment with B. subtilis mutants. From the total results of this research, it can be concluded that the induction of mutation with gamma ray due to the improvement of antagonistic potential of B. subtilis ,leaded to increase the biological control activities of B. subtilis mutants without adverse effects on the growth and physiology of lettuce, so that the efficiency of these mutants in the biocontrol of pseudo-fungus P. ultimum and induction of physiological indicators of resistance in the lettuce plant is higher than that of Azosprilium efficiency.

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References

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