Green Synthesis of Iron Oxid Nanoparticles Using Cressa Cretica leaf Extract and Investigating its Antimicrobial Efficacy

Sarani, Azim; Mehravaran, Leila; Fakheri, Barat Ali; Allahdou, Maryam

doi:10.30473/cb.2025.71377.1969

Green Synthesis of Iron Oxid Nanoparticles Using Cressa Cretica leaf Extract and Investigating its Antimicrobial Efficacy

Articles in Press

Document Type : Research Paper

Authors

¹ Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Zabol, Zabol, Iran

² Assistant Prof, Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Zabol, Zabol, Iran

³ Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Zabol University, Zabol, Iran

10.30473/cb.2025.71377.1969

Abstract

In order to green synthesis of iron nanoparticles, the leaf extract of Cressa cretica L. was used as a reducing agent. In order to green synthesize iron oxide nanoparticles, the extract of Cressa cretica L. was used as a reducing agent. Within a few minutes of adding iron sulfate to leaf the extract of Cressa cretica L., iron oxide nanoparticles were formed. The first sign of the synthesis of nanoparticles was the change in the color of the solution from yellow to dark brown. The synthesized nanoparticles were analyzed by various techniques such as ultra-violet spectrophotometry, XRD, FT-IR, TEM. In the spectrum obtained from the UV-spectrophotometer, the peak appeared at 280 nm, which indicates the transfer of oxygen electrons to iron synthesized from Cressa. The XRD spectrum depicted the crystal structure of the synthesized iron oxide nanoparticles. The uniform spherical particles of the synthesized iron oxide nanoparticles with an average size of 21 nm were visible using TEM images. The FT-IR spectrum shows the presence of functional groups of plant chemicals that probably play a role in the formation and stabilization of nanoparticles. These results confirm the presence of flavonoid and phenolic compounds in leaf extract. These compounds can be responsible for the reduction of metal ions and the formation of nanoparticles. Synthetic iron oxide nanoparticles showed effective inhibition against Staphylococcus aureus, These results indicate the possible effective use of synthetic nanoparticles in the production of antibacterial drugs and various biological applications.

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