Document Type : Research Paper
Authors
1
1. M.Sc,, Department of Plant Biotechnology, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran.
2
Associate Professor, Department of Agricultural Biotechnology, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran.
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3. Associate Prof. Agricultural Biotechnology Research Institute of Iran (ABRII), North Region Branch, Agricultural Research, Education and Extension Organization (AREEO), Rasht, Iran
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4. Professor, Department of Plant Biotechnology, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran. E-mail address: hsamizadeh@guilan.ac.ir
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5. Instructor. Agricultural Biotechnology Research Institute of Iran (ABRII), North Region Branch, Agricultural Research, Education and Extension Organization (AREEO), Rasht, Iran
Abstract
To this end, the expression of glutathione reductase (GR), thioredoxin (TRX), and glutaredoxin (GRX), was assessed in two genotypes of Rapeseed: the sensitive variety (Hyola 308) and the tolerant variety (SLM046) under drought stress (withholding irrigation for 72 hours), a 20% (v/v) methanol spraying treatment, and control conditions (continuous irrigation). This was conducted using the Real-Time PCR technique. Sampling was performed at the 4-6 leaf stage at 0, 8, and 24 hours after the stress was applied.Morphological observations showed that the rapeseed tolerant and sensitive genotypes regained their freshness after spraying methanol under drought stress (interruption of irrigation). The results indicate that the highest increase in glutathione reductase gene expression in the Hyola 308 variety occurred eight hours after the application of stress without methanol treatment. In contrast, the SLM 046 variety exhibited initially low expression levels during the early hours of stress, which increased significantly with methanol treatment, registering almost a fourfold increase. The expression level of the thioredoxin gene in the Hyola 308 variety peaked 8 hours after the drought stress with methanol treatment, indicating that methanol application enhanced gene expression and plant resistance. In the SLM 046 variety, thioredoxin gene expression gradually increased after the application of stress, persisting up to 24 hours post-stress. However, the gene expression initially decreased with methanol treatment but eventually reached its maximum after 24 hours. For glutaredoxin gene expression, the Hyola 308 variety showed lower levels 8 hours after the drought stress without methanol treatment compared to the baseline (0 hours).
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