Primary field evaluation of transgene in some cotton transgenic lines against Lepidoptera pests

Mortazavi, ُSayyed-Elyass; Arabsalmani, Morteza

doi:10.30473/cb.2025.72568.1986

Primary field evaluation of transgene in some cotton transgenic lines against Lepidoptera pests

Document Type : Research Paper

Authors

¹ Agricultural Biotechnology Research Institute of Iran, Agricultural Research, Extension and Education (AREEO), Karaj, PO Box: 31535-1897

² Tehran Agricultural and Natural Resources Research Center, Agricultural Research, Extension and Education (AREEO), Qoddousi Boulvard, Varamin, Tehran

10.30473/cb.2025.72568.1986

Abstract

In order to evaluate characteristics related to Lepidoptera pest resistance in some transgenic cotton lines, different transgenic events from Cocker100 variety harbouring cryIAb gene were tested in a field experiment. The other plant materials were consisted from two backcrossed lines resulted from MON531 event and CIM-448 line, and a Chinese commercial transgenic cotton cultivar harbouring cryIAc gene named 11F1 as positive control, and Khordad and Varamin cultivars as the non-transgenic negative control were used in the experiment. A randomaized complete block design with three replications was adopted as the statistical model. Infestation of the field during growing season was take palce in six stages to ensure that the field was infected uniformly by the pest. The characters related to the pest resistance trait were recorded during growing season. The results showed that non-transgenic cultivars comprising Varamin and Khordad experienced 24.7 and 23 percent respectively, the maximum boll contamination, while transgenic lines showed a contamination range from relatively suseptable (%19) to relative resistance (%2.5). Also, Maximum damage index of the pest were exhibited in the Varamin and Khordad cultivars with %6.4 and %5.9 respectively, while the index for transgenic lines varied from %5 to %0.6. In general, the transgenic A and B lines showed acceptable bollworm resistance characters. More investigations are needed to confirm other agronomic and fiber quality to be considered in commercialization of the lines.

Keywords

Main Subjects

Genetic Engineering and Gene Transformation

References

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