Comparative Analysis of Nodulation and Gene Expression in Different Chickpea Genotypes Following Inoculation with Mesorhizobium ciceri

Document Type : Research Paper

Authors

Department of Genetic and Plant Production, University of Kurdistan

10.30473/cb.2025.74301.2007

Abstract

In this study, nodulation capacity and physiological traits were evaluated in ten chickpea (Cicer arietinum) genotypes following inoculation with Mesorhizobium ciceri. After comparing traits such as nodule number, nodule weight, total fresh weight, total dry weight, stem dry weight, root dry weight, stem length, and root length, two genotypes (Bionij and Pirooz) were selected for further investigation as the most and least efficient in symbiosis, respectively. Flavonoids play a crucial role as initial signaling molecules in the symbiotic interaction between legumes and nitrogen-fixing bacteria. Subsequently, the quantitative expression of the Isoflavone 4'-O-methyltransferase gene, involved in the isoflavonoid biosynthesis pathway, was assessed in the two chickpea genotypes (Bionij and Pirooz) at three time points post-inoculation (36 hours, 10 days, and 28 days) using Real-Time PCR. The results indicated a significant upregulation of this gene in both genotypes, particularly at 36 hours post-inoculation. This increase was more pronounced in the Bionij genotype. Leaf chlorophyll content and nitrogen levels in nodules and roots were measured in both treated and control plants. Data revealed that treated plants exhibited significantly higher levels of chlorophyll and nitrogen, with Bionij outperforming Pirooz in both parameters. These findings suggest that higher expression of this gene may be associated with enhanced nodulation capacity, improved symbiosis, and increased nitrogen uptake in chickpea.

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Main Subjects


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