Document Type : Research Paper
Authors
Gorgan agricultural and natural resources
Abstract
Maize dwarf mosaic virus is one of the most important and most extensive viruses of maize in the world. In this paper investigated molecular comparative of nucleotide sequence of coding regions of proteins of 5΄ region of the genome of Maize dwarf mosaic with the other isolate and other cereal potyviruses. In order to this, maize plants with mosaic symptoms and dwarfing were collected from Golestan province. After verification of plants by Elisa test against MDMV antiserum, an infected sample was selected for isolation of RNA. Amplification of the 5΄ region of the genome has been conducted using five specific primer pair that was obtained 5 overlap segments including P1, HC-Pro ، p3 و 6k1genes, as well as 5΄-UTR region of the genome and was obtained 3460bp. The comparative analysis of nucleotide sequences of MDMV strains showed that the Iranian (Golestan) isolate have 95.7 and 92.3 percent similarity at the amino acid level and 90.5 and 85.1 percent at the nucleotide level with Bulgarian and Spanish isolates respectively, and with other homogeneous viruses, MDMV had similarity maximum with sorghum mosaic virus from Texas and sugarcane mosaic virus from china 72.9 and 72.3 percent respectively.
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