Tobacco Transformation by FsTri101 Gene Encoding Acetyltransferase from Fusarium sporotrichioides and Study of Its Effect in Detoxification of Deoxynivalenol Trichothecene

Banaee, Mahsa; Sanjarian, Forogh; Bakhshi Khaniki, Gholam Reza

Tobacco Transformation by FsTri101 Gene Encoding Acetyltransferase from Fusarium sporotrichioides and Study of Its Effect in Detoxification of Deoxynivalenol Trichothecene

Document Type : Research Paper

Authors

¹ M.Sc. of Agricultural Biotechnology, Payame Noor University, Tehran, Iran;

² Assistant Professor, Department of Plant Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran

³ Department of Agricultural Biotechnology, Payame Noor University, Tehran, Iran

Abstract

Acetyl transferases are enzymes responsible for enzymatic transfer of an acetyl group to suitable receptor molecule by using acetyl CoA as donor. Acetyltransferase reaction is involved in biosynthesis pathway of some important secondary metabolites such as antibiotics, as well as in their detoxification. Trichothecenes are significant secondary metabolites produced by the plant fungal phatogens Fusarium ssp. Such as F. sporotrichioides and F. graminearums. These fungi possess specific genes in their genomes encodeinge acetyl transferase enzymes are affecting trichothecene. In this study, the gene encoding acetyltransferase from the fungus F. sporotrichioides, TRI 101, was cloned and transferred into tobacco plant as model plants and the effect of the enzyme on the detoxification of deoxynivalenol (DON), a well known trichthecene, was investigated. In addition it was shown that, in comparison whit the roots of wild type plants, transgenic roots grew normally in the deoxynivalenol-contained medium.

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