Document Type : Research Paper
Authors
1 M.Sc. of Agricultural Biotechnology, Sari Agricultural Sciences and Natural Resources University, Sari, Iran
2 M.Sc. of Plant Cellular Developmental Biology, Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran
3 M.Sc. of Agricultural Biotechnology, Sari Agricultural Sciences and Natural Resources University, Sari, Iran.
Abstract
Using Agrobacterium rhizogenes to induce hairy root cultures is a useful method to increase the production of secondary metabolites especially medicinal compounds in vitro in various plant species. Hairy root cultures are fast growing and highly branching and due to the higher amount of metabolites synthesized per unit of biomass, possess the same or greater biosynthetic potential for secondary metabolite production compared to the normal roots. In this research the amounts of total phenolics and chlorogenic acid were determined in hairy root clones induced in cotyledonary leaves of Cichorium intybus by A4, A13 and 15834 strains of A. rhizogenes, as well as in the control (untransformed) roots. The results obtained indicated that the absence of chlorogenic acid in all the studied clones led to a significant increase in total phenolics in hairy root clones induced by A4 and A13 but a significant decrease in the 15834-induced hairy root. This study revealed the role of bacterial strains in biosynthesis of phenolic compounds, and therefore, selection and application of the best strain of A. rhizogenes could be regarded as an important strategy for increasing phenolic compounds production in hairy root cultures of C. intybus.
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