Crop Biotechnology

In collaboration with Payame Noor University and Iranian Biotechnology Society

Current Issue

By Issue

By Author

By Subject

Author Index

Keyword Index

About Journal

Aims and Scope

Editorial Board

Publication Ethics

Indexing and Abstracting

Related Links

FAQ

Peer Review Process

Journal Metrics

News

Genetic diversity and association analysis for morphophenolgic traits and resistance to Powdery mildew using ISSR, IRAP and iPBS markers

Document Type : Research Paper

Authors

1 M.Sc. Student of Agricultural Biotechnology, Gonbad Kavous University, Gonbad Kavous, Iran

2 Assocate Professor, Department of Plant Production, Faculty of Agriculture and Natural Resources, Gonbad Kavous University, Gonbad Kavous, Iran.

3 Assistant Professor, Department of Plant Production, Faculty of Agriculture and Natural Resources, Gonbad Kavous University, Gonbad Kavous, Iran.

4 Academic member, Seed and Plant Improvement Research Department, Golestan Agricultural and Natural Resources Research and Education Center, AREEO, Gorgan, Iran.

Abstract

In order to evaluation of genetic diversity and association analysis of morpho-phonological traits and mildew disease of wheat germplasm, 115 wheat genotypes were planted in the research field of Gonbad Kavous University as RCBD design with 3 replications in 2015-16. According to the results, there was a significant difference between all measured traits except grain length. In order to evaluation of molecular diversity in 60 wheat genotypes, 8 ISSR, IPBS and IRAP primers were used. Out of 61 bands produced in total, 47 bands were polymorph. The number of polymorphic bands was different range from 2 to 14 bands for each primer. The maximum percent of polymorphism with %100 polymorphism belonged to PRI-46 primer, and the minimum percent of polymorphism with %50 polymorphism belonged to PRI-59, PRI -10 and PRI-5 primers. In this study, also 60 genotypes and lines of wheat were evaluated in-vitro against Bgt. Cluster analysis of data of Powdery mildew caused was grouped lines in three groups of sensitive, resistance and median using UPGMA algorithm and Euclidian distance. Mean comparison using LSD indicated that lines 127 and 801 were high resistance but lines 390, 515 and 833 were susceptible to Bgt. Association analysis between markers and traits morpho-phenological showed that a total of 56 alleles showed a connection between the characters and PR50-6 allele associated most relevant to the characters. Based on results of this research, the higher genotypes could be screened for advanced wheat breeding steps.

Keywords

Main Subjects

References
Alam MA, Wang H (2011) Powdery Mildew Resistance Genes in Wheat: Identification and Genetic Analysis. J. Mol. Biol. 1(1): 20-39.
An ZW, Xie LL, Cheng H, Zhou Y, Zhang Q, and He XG (2009) A silver staining procedure for nucleic acids in polyacrylamide gels without fixation and pretreatment. Analytical Biochemistry. Aug. 391(1):77-9.
Arzani A (2002) Grain yield performance of durum wheat germplasm under Iranian dry land and irrigated field conditions. Sabrao J. Breed. Genet. 34: 9-18.
Ashrafi Parchin R (2011) Evaluation of genetic diversity of wheat (Triticum aestivum) genotypes using agronomic and morphological characters and molecular markers. M. Sc. Thesis, University of Razi, Kermanshah, Iran. (in Persian)
Barker JSF (1999) A global protocol for determining genetic distances among domestic livestock breeds. Proceeding of the 5th world congress on Genetics Applied to Livestock productions. University of Guelph, Guelph. 21: 501-508.
Byun SO, Fang Q, Zhou H, and Hickford J.G (2009) An effective method for silver-staining DNA in large numbers of polyacrylamide gels.  Analytical Biochemistry. 385(1): 174-5.
Carvalho A, Lima-Brito J, Macas B, and Guedes-Pinto, H (2009) Genetic diversity and variation among botanical varieties of old Portuguese wheat cultivars revealed by ISSR assays. Biochem. Genet. 47: 276-294.
Gradzielewska A (2012) Identification of hybrids between triticale and aegilops juvenalis (Thell) eig and determination of Jeuetic similwity with ISSR. Genet. Mol. Res. 13.11(3): 2147-55.
International Rice Genetics Symposium, Manilla, Philippines, 16-20 October: 307-316.
Kölliker R, Jones ES, Drayton MC, Dupal MP, Forster JW (2001) Development and characterization of simple sequence repeat (SSR) marker for white clover (Trifolium repens L.). Theor. Appl. Genet. 102: 416-424.
Kraakman ATW, Niks RE, Van den berg PMMM, Stam P, Van Eeuwijk FA (2004) Linkage disequilibrium mapping of yield and yield stability in modern spring barley cultivars. Genetics 168(1): 435-46.
Kumar A, Hirochika, H (2001) Applications of retrotransposons as genetic tools in plant biology. Trend Plant Sci. 6(3).13.
 Louis E, Dempte E (1987) An exact test for Hardy-Weinberg and multiple alleles. Biometrics 43(4): 805-11.
Maas EV, Lesch SM, Francois LE, Grieve CM (1996) Contribution of individual culms to yield of salt-stressed wheat. Crop Sci. 36: 142-149.
Najaphy A, Ashrafi Parchin R, Farshadfar E (2011) Evaluation of genetic diversity in wheat cultivars and breeding lines using inter simple sequence repeat markers. Biotech. Biotech. Equip. 10: 2634-2638.
Nevo E (1978) Genetic variation in natural populations: Patterns and Theory. T.P.B. 13: 121-127.
Norkhalaj M, Khodarahmi M, Amini A, Esmaielzade M, Sadegh Moghadam R (2010) Study on Correlation and Causation relations of Morphological traits in synthetic wheat liens. J. Agron. Plant Breed. 6(3): 7-17.
Pierre CS, Crossa J, Manes Y, Reynolds MP (2010) Gene action of canopy temperature in bread wheat under diverse environments. Theor. Appl. Genet. 120: 1107–1117.
Ramiz Tagi A, Mehraj AA, Alamdar CM (2007) Genetic identification of diploid and tetraploid wheat species with RAPD markers. Turk. J. Biol. 31:173-180.
Roldan-Ruiz FA, Gilliland TJ, Dubreuil P, Dillmann C, Lallemand J (2001) A comparative study of molecular and morphological methods of describing relationships between perennial ryegrass (Lolium perenne L.) varieties. Theor. Appl. Genet. 103: 1138-1150.
Saghi Maroof MA, Biyaoshev RM, Yang GP, Zhang Q, Allard RW (1994) Extra ordinarily polymorphic microsatellites DNA in barly species diversity, chromosomal location, and population dynamics. Processing of the academy of sciences, USA. 91: 4566-5570.
Salari M, Yazdani D, Okhovvat M, Akbari A (2002) Evaluation of resistance of some wheat cultivars to powdery mildew in Mazandaran. Appl. Entomology. Phytopathology 70: 25-36.
Shannon CE, Weaver W (1949) The Mathematical Theory of Communication. University of Illinois Press: Urbana, USA.
Syed Aghamiri SMM, Mostafavi Kh, Mohammadi A (2010) Relationships between yield and yield components under normal and drought stress in barley genotypes using path analysis. The 5th conference of new ideas in agriculture, 1-3pp.  Azad University of Khorasan, Iran.
VanBeuningen LT, Busch RH (1997) Genetic diversity among North American spring wheat cultivars: Analysis of the coefficient of parentage matrix. Crop Sci. 37: 570-579.
Vanda M, Hoshmand S (2011) Genetic analysis of grain yield and related traits in durum genotypes using diallel. Iranian J. Crop Sci. 13(1): 206-218.
Virk PS, Ford-Lioyd BV, Jackson MT, Pooni HS, Clemeno TP, Newbury HJ (1996) Marker-assisted prediction of agronomic traits using diverse rice germplasm. In: IRRI, Rice genetics III, Proceedings of The Third International Rice Genetics Symposium, Manilla, Philippines, 16-20 October: 307-316.
Zhu YF, Hu J, Han R, Wang Y, Zhu S (2011) Fingerprinting and identification of closely related wheat (Triticum aestivum L.) cultivarsusing ISSR and fluorescence-labeled TP-M13-SSR markers. Aust. J. Crop Sci. 5(7): 846-850.
Crop Biotechnology
Volume 7, Issue 18 - Serial Number 18
November 2017
Pages 41-56
Files
Share
How to cite
Statistics