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Allelic variation, Association Analysis and haplotype diversity for microsatellite markers related to acidic soil tolerance genes in barely

Document Type : Research Paper

Authors

1 M.Sc. student of Biotechnology Crop, Department of Plant Production, Faculty of Agriculture and Natural Resources, University of Gonbad Kavous, Iran.

2 Associate Professor, Department of Plant Production, Faculty of Agriculture and Natural Resources, University of Gonbad Kavous, Iran.

3 Assistant Professor, Department of Plant Production, Faculty of Agriculture and Natural Resources, University of Gonbad Kavous, Iran.

4 Assistant Professor, Department of Plant Breeding Research and Preparation of Seed and Seedlings, Golestan Agricultural and Natural Resources Research and Education Center, Education and Agricultural extension, Gorgan, Iran.

Abstract

Acidic soils are a serious threat to the production of crops throughout the world. In order to study the alleles associated with soil acidity tolerance in barley plant, and also accomplish effective haplotype groups, phenotypic testing in pots containing acidic soil and in the form of Augment with 96 genotypes and 4 controls, and 27 traits were evaluated on plants. To investigate the allelic and haplotypic diversity, the genotypes were identified by 7 microsatellite markers related to soil acidity tolerance. Analysis of allelic variation showed that the highest pic and Gene Diversity were 3.429, 0.441 and 0.490 respectively, which belonged to the HvMATE-21indel marker, and the Cit7 marker had the lowest value. Also, the results of the haplotype study showed 41 haplotype groups. The group 16, which included genotype 6, had the highest yield and soil acidity resistance with a yield of 2.017 gr / plant. association analysis between molecular and phenotypic data suggested that among 20 effective alleles on the evaluated traits, the Do-D allele had the greatest impact on yield and its components with effect on 3 traits, number of seeds per spike, number of Fertile spike and yield (in plant). Do-B was also R2 equal to 39.5 for the number of spikes per plant of the highest R2 among the alleles involved in yields and yield components. The markers associated with tolerant haplotypes and tolerant genotypes in this study can be used in research and breeding programs.

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Crop Biotechnology
Volume 8, Issue 22
September 2018
Pages 27-39
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