Crop Biotechnology

In collaboration with Payame Noor University and Iranian Biotechnology Society

Current Issue

By Issue

By Author

By Subject

Author Index

Keyword Index

About Journal

Aims and Scope

Editorial Board

Publication Ethics

Indexing and Abstracting

Related Links

FAQ

Peer Review Process

Journal Metrics

News

The effect of iron stress on the relative expression level of SOD, MDHR and GPX1 genes in tolerant and sensitive genotypes of Rice

Document Type : Research Paper

Authors

1 Assistant Professor, Department of Agricultural Biotechnology, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran.

2 M.Sc., Department of Agricultural Biotechnology, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran.

3 Ph. D, Department of Agricultural Biotechnology, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran.

4 Professor, Department of Agricultural Biotechnology, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran.

5 Instructor, Department of Agricultural Biotechnology, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran.

Abstract

Increasing of reactive oxygen species (ROS) under iron toxicity is considered as one of the major constraints to rice production. In this study the alterations of SOD, GPX1 and MDHR expression level in two genotypes of rice, Pokkali (as tolerant) and IR64 (as sensitive) were monitored under different concentrations of iron levels [(0) (nonstress)], 100, 250, 400 and 500 mg/lit-1Fe-EDTA). The treatments were done when the plants were at 4-leaf stage and lasted for two weeks. Results showed that the expression levels of genes in Pokkali were higher than IR64. The expression level of SOD in IR64, increased at iron concentration increased, while it decreased at higher Fe-level. The expression level of GPX1 was increased in IR64, but decreased in Pokkali. The expression level of MDHR in IR64 was decreased at early stage of Fe-treatment, but then increased. Inversely, in Pokkali MDHR expression reduced constantly under Fe stress. Overall, the relative over expression of genes in Pokkali and presence of different expression levels of them between different concentrations of Fe in tolerant and sensitive genotypes indicate that the gene could remarkably effect on the tolerant level of pokkali by reducing ROS production under Fe-toxicity.

Keywords

Main Subjects

References
Alscher RG, Erturk N, Heath LS (2002) Role of superoxide dismutases (SODs) in controlling oxidative stress in plants. Journal of Experimental Botany. 53(372): 1331-1341.
Asada K (1999) The water-water cycle in chloroplasts: scavenging of active oxygens and dissipation of excess photons. Annual Review of Plant Biology. 50(1): 601-639.
Becker M, Asch F (2005) Iron toxicity in rice-condition and management concepts. Journal of Plant Nutrition and Soil Science. 168: 558-573.
Birla DS, Malik K, Sainger M, Chaudhary D, Jaiwal RK, Jaiwal P (2015) Progress and challenges in improving the nutritional quality of rice (Oryza sativa). Journal of Critical Reviews in Food Science and Nutrition. 57(11): 2455-2481.
Bowler C, Montagu MV, Inze D (1992) Superoxide dismutase and stress tolerance. Annual Review of Plant Biology. 43(1): 83-116.
Diao Y, Xu H, Li G, Yu A, Yu X, Hu W (2014) Cloning a glutathione peroxidase gene from Nelumbo nucifera and enhanced salt tolerance by overexpressing in rice. Journal of Molecular Biology Reports. 41: 4919-4927.
Dorak M (2007) Real-time PCR. Taylor, Francis Group Press. 333.
Dufey I, Hakizimana P, Draye X, Lutts S, Bertin P (2009) QTL mapping for biomass and physiological parameters linked to resistance mechanisms to ferrous iron toxicity in rice. Journal of Euphytica. 167(2): 143-160.
Elstner EF (1991) Mechanisms of oxygen activation in different compartments of plant cells. Journal of Food and Agriculture Organization of the United Nations. 13-25.
Fahey RC, Sundquist AR (1991) Evolution of glutathione metabolism. Journal of Advances in Enzymology. 64: 1-53.
Faize M, Burgos L, Faize L, Piqueras A, Nicolas E, Barba-Espin G, Clemente-Moreno M, Alcobendas R, Artlip T, Hernandez J (2011) Involvement of cytosolic ascorbate peroxidase and Cu/Zn-superoxide dismutase for improved tolerance against drought stress. Journal of Experimental Botany. 62(8): 2599-2613.
Fu J, Huang B (2001) Involvement of antioxidant and lipid peroxidation in the adaptation of two cool-season grasses to localized drought stress. Journal of Environment and Experimental Botany. 45: 105-114.
Fu JY (2014) Cloning of a new glutathione peroxidase gene from tea plant (Camellia sinensis) and expression analysis under biotic and abiotic stresses. Journal of Botanical studies. 55(1): 7.
Gao H, Liu CP, Song SQ, Fu J (2016) Effects of dietary selenium against lead toxicity on mRNA levels of 25 selenoprotein genes in the cartilage tissue of broiler chicken. Journal of Biological Trace Element Research. 172(1): 234-241.
Gupta AS, Heinen JL, Holaday AS, Burke JJ, Allen RD (1993) Increased resistance to oxidative stress in transgenic plants that overexpress chloroplastic Cu/Zn superoxide dismutase. Journal of Proceedings of the National Academy of Sciences. 90(4): 1629-1633.
Hell R, Stephan UW (2003) Iron uptake, trafficking and homeostasis in plants. Journal of Planta. 216: 541-551.
Herbette S, de Labrouhe DT, Drevet JR, Roeckel-Drevet P (2011) Transgenic tomatoes showing higher glutathione peroxydase antioxidant activity are more resistant to an abiotic stress but more susceptible to biotic stresses. Journal of Plant Sciences. 180: 548-553.
Huseynova IM, Aliyeva DR, Aliyev JA (2014) Subcellular localization and responses of superoxide dismutase isoforms in local wheat varieties subjected to continuous soil drought. Journal of Plant Physiology and Biochemistry. 81: 54-60.
Jiménez A, Hernandez JA, Del Rio LA, Sevilla F (1997) Ascorbate-glutathione cycle in mitochondria and peroxisomes of pea leaves: changes induced by leaf senescence. Journal of Phyton-Horn. 37:101-108.
Jin J, Shan N, Ma N, Bai J, Gao J (2006) Regulation of ascorbate peroxidase at the transcript level is involved in tolerance to postharvest water deficit stress in the cut rose (Rosa hybrida L.) cv. Samantha. Journal of Postharvest Biology and Technology. 40(3): 236-243.
Kang GZ, Li GZ, Liu GQ, Xu W, Peng XQ, Wang CY, Zhu YJ, Guo TC (2013) Exogenous salicylic acid enhances wheat drought tolerance by influence on the expression of genes related to ascorbate-glutathione cycle. Journal of Biologia Plantarum. 57(4): 718-724.
Kuroda H, Sagisaka S, Chiba K (1992) Collapse of peroxide-scavenging systems in apple flower-buds associated with freezing injury. Journal of Plant and Cell Physiology. 33(6): 743-750.
Kwon SY, Jeong YJ, Lee HS, Kim JS, Cho KY, Allen RD, Kwak SS (2002) Enhanced tolerances of transgenic tobacco plants expressing both superoxide dismutase and ascorbate peroxidase in chloroplasts against methyl viologen‐mediated oxidative stress. Plant, Cell and Environment. 25(7): 873-882.
Lee SH, Ahsan N, Lee KW, Kim DH, Lee DG, Kwak SS, Kwon SY, Kim TH, Lee BH (2007) Simultaneous overexpression of both CuZn superoxide dismutase and ascorbate peroxidase in transgenic tall fescue plants confers increased tolerance to a wide range of abiotic stresses. Journal of Plant Physiology. 164(12): 1626-1638.
Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2− ΔΔCT method. Journal of Methods. 25(4): 402-408.
Mehraban P, Abdol Zadeh A, Sadeghipour HR (2008) Iron Toxicity in Rice (Oryza sativa L.), under Different Potassium Nutrition. Asian Journal of Plant Sciences. 7(3): 251-259.
Mehraban P, Abdolzadeh A (2011) Effects of iron toxicity on growth four rice varieties highlighted some biochemical aspects in resistant variety.  Eectronic Journal Crop Production. 4(3): 103-120.
Meister A (1994) Glutathione, Ascorbate, and Cellular Protection. Journal of Cancer Research. 4:1969-1975.
Ozden M, Demirel U, Kahraman A (2009) Effects of proline on antioxidant system in leaves of grapevine (Vitis vinifera) exposed to oxidative stress by H2O2. Journal of Scientia Horticulturae. 119(2): 163-168.
Pasandideh Arjmand M, Samizadeh Lahiji H, Mohsenzadeh Golfazani M (2017) The investigation of some photorespiration genes relative expression in response to drought stress in canola (Brassica napus). Journal of Crop Biotechnology. 7(17): 31-42.
Passaia G, Fonini LS, Caverzan A, Jardim-Messeder D, Christoff AP, Gaeta ML (2013) The mitochondrial glutathione peroxidase GPX3 is essential for H2O2 homeostasis and root and shoot development in rice. Journal of Plant Sciences. 208: 93-101.
Pazoki A, Rad AS, Habibi D, Paknejad F, Kobraee S, Hadayat N (2010) Effect of drought stress and selenium spraying on superoxide dismotase activity of winter rapeseed (Brassica napus L.) cultivars. World Academy of Science, Journal of Engineering and Technology. 44: 688-691.
Perl A, Perl-Treves R, Galili S, Aviv D, Shalgi E, Malkin S, Galun E (1993) Enhanced oxidative-stress defense in transgenic potato expressing tomato Cu, Zn superoxide dismutases. Journal of Theoretical and Applied Genetics. 85(5): 568-576.
Saeedfar S, Negahban M, Soorestani MM (2015) The Effect of Drought Stress on the Essential Oil Content and Some of the Biochemical Characteristics of Anise Hyssop (Agastache foeniculum [Pursh] Kuntze). European Journal of Molecular Biotechnology. 8(2): 103-114.
Sairam R, Chandrasekhar V, Srivastava G (2001) Comparison of hexaploid and tetraploid wheat cultivars in their responses to water stress. Journal of Biologia Plantarum. 44(1): 89-94.
Sales CR, Ribeiro RV, Silveira JA, Machado EC, Martins MO, M Lagôa AM (2013) Superoxide dismutase and ascorbate peroxidase improve the recovery of photosynthesis in sugarcane plants subjected to water deficit and low substrate temperature. Journal of Plant Physiology and Biochemistry. 73: 326-336.
Smirnoff NE (2008) Antioxidants and reactive oxygen species in plants. John Wiley & Sons.
Takahashi MA, Asada K (1983) Superoxide anion permeability of phospholipid membranes and chloroplast thylakoids. Journal of Archives of Biochemistry and Biophysics. 226: 558-566.
Van Camp W, Capiau K, Van Montagu M, Inze D, Slooten L (1996) Enhancement of oxidative stress tolerance in transgenic tobacco plants overproducing Fe-superoxide dismutase in chloroplasts. Journal of Plant Physiology. 112(4): 1703-1714.
Wu LB, Shhadi MY, Gregorio G, Matthus E, Becker M, Frei M (2014) Genetic and physiological analysis of tolerance to acute iron toxicity in rice. Journal of Rice. 7(1): 8.
Yoshida S, Fomo DA, Cock JH (1976) Laboratory manual for physiological studies of rice. Los Banos (Philipines) International Rice Research Institute.
Crop Biotechnology
Volume 8, Issue 24
December 2018
Pages 1-13
Files
Share
How to cite
Statistics