Document Type : Research Paper
Authors
- Mohsen Esmailzadeh Moghadam 1
- Fatemeh Samadi Khozani 2
- Agha Fkhar Mirlohi 3
- Badredin Ebrahim Tabatabaie 3
1 Associate Professor, Seed and Plant Improvement Institute, Karaj, Iran.
2 M.Sc. of Plant Breeding, Isfahan University of Technology, Isfahan, Iran,
3 Professor, Faculty of Agriculture, Isfahan University of Technology, Isfahan, Iran.
Abstract
Hulled wheats are among the earliest domesticated Triticeae. They possess many important agronomic traits such as tolerance to biotic and abiotic stresses , higher grain protein quality and quantity, and higher micronutrients concentration. These characteristics has made hulled wheat a useful germplasm resource and highly valuable in wheat breeding programs. Hulled wheats are found in different regions of Iran but their genetic potential is not exploited owing to lack of genomic information. In this research, two hulled wheat accessions (Zarne and Jonghan) collected from farmers field in two distantly located villages in the Chehar-Mahal Bakhtiari province with other 28 diploid, tetraploid and hexaploid wheat genotypes were studied using seventeen Simple Sequence Repeats (SSR) primers. The SSR markers used were related to the A and B genomes of wheat and generated between 2 to 11 alleles per locus with the average polymorphism information content (PIC) of 0.53 per locus. The high value of PIC showed that a high level of polymorphism was present for accessions classification. The dendrogram derived from SSR data clustered two accessions of hulled wheat (Zarne and Jonghan) with two T. dicoccoides (wild emmer) in a single group, while T. dicoccum (domesticated emmer) appeared in a distantly related class. The three T. durum (free-threshing) were placed in a separate cluster, distant from T. dicoccum. These results showed that Zarne and Jonghan genotypes have closer affinity to T. dicoccoides than T. dicoccum genotypes.
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