Document Type : Research Paper
Authors
1 M.Sc. Gradated, Department of Agronomy and Plant Breeding, Faculty of Agriculture, Shahrekord University, Shahrekord, Iran.
2 M.Sc. Student, Department of Agriculture Biotechnology, Faculty of Agriculture, Shahrekord University, Shahrekord, Iran.
3 Assistant Professor, Department of Agronomy and Plant Breeding, Faculty of Agriculture, Shahrekord University, Shahrekord, Iran.
4 Associate Professor, Department of Genomics, Agricultural Biotechnology Research Institute of Iran, Karaj, Iran.
Abstract
Bread wheat (Triticumaestivum) is one of the most important world food crops that are exposed to many pathogen. During the previous expression-profiling experiments, in addition to major resistant genes to disease in wheat, some defense-related genes such as S-Like RNase gene have been identified. Here to study expression pattern of this gene in several fungal wheat diseases, some bioinformatics and laboratory studied were performed. In bioinformatics studies, several microarray libraries infected with Fusarium, Spike blight and Stripe rust were considered. In laboratory experiments Septoria tritici bloth was studied. So the level of expression was measured at 8 time interval, from 0h to 6 days after inoculation by Mycosphaerella graminicola in Wangshuibai as a resistant wheat cultivar and Falat as a susceptible wheat cultivar by semi quantitative reverse transcriptase-polymerase chain reaction (RT-PCR). The results show that the maximum expression of this gene, depending on types of disease and resistant cultivars, is obtained up to 24 hours after the inoculation. Thus, according to this results it can be concluded that this gene plays an important role in resistance to diseases and ,along with the main gene, increase and maintain resistance to many fungal diseases in wheat. Also this gene was confirmed by Nucleotide Blast.
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