Evaluation of genetic diversity among hull-less and hulled rain-fed barley genotypes using SSR molecular marker

Esmaili, Ahmad; Talebi, Behnaz; Drikvand, Reza; Ebrahimi, Mohammad Ali; Hossienpour, Tahmasb

Evaluation of genetic diversity among hull-less and hulled rain-fed barley genotypes using SSR molecular marker

Document Type : Research Paper

Authors

¹ Assistant Professor, Department of Crop Science and Plant Breeding, Lorestan University, Khorramabad, Iran

² Graduate of M.Sc., Payame Noor University, Tehran, Iran,

³ Assistant Professor, Department of Crop Science and Plant Breeding, Islamic Azad University Khorramabad branch, Khorramabad, Iran,

⁴ Associate Professor, Department of Agricultural Biotechnology, Payame Noor University, Tehran, Iran,

⁵ Member of Scientific Board of Agriculture and Natural Resources Research Center of Lorestan Province, Khorramabad, Iran.

Abstract

In order to study of genetic diversity of barley genotypes, 14 pair’s primers of SSR were used in 20 genotypes. After genomic DNA extraction and PCR reaction, Primers produce 266 polymorph bands and mean of polymorph band per primer was 19. The highest value of polymorphic information content (PIC) belonged to Hvm60 and Hvm20 primers (0.88 and 0.73, respectively) and the lowest value of PIC was belonged to Bmac0032 primer (0.32) and mean of PIC for all primers were 0.6. Jacard similarity coefficient was calculated and ranged from 0.3 to 0.96 among genotypes. The highest genetic similarity (0.96) belonged to genotypes no. 6 and 5 and the lowest (0.3) was belonged to genotypes no. 13 and 10. Clustering dendrogram based on UPGMA method classified genotypes to 5 main groups. Results of PCOA classification were similar to results of cluster analysis. Evaluation of genotypes by SSR molecular marker could discriminate two row and six row genotypes and also hulled and hulless genotypes. In other hand the similar parents in pedigree of some genotypes (e.g. between 3 and 12 and between 14 and 18) had an important effect on this classification. Results of this study revealed that this molecular marker has a valuable potential for evaluation and discrimination of barley genotypes.

Keywords