Document Type : Research Paper
Authors
1 Assistant professor and Research Deputy of Agricultural and Natural Resources Research Center of Zanjan Province
2 Alghadir Technical College, Zanjan and M.Sc. Graduated of Agricultural Biotechnology, Payame Noor University, Iran,
3 Assistant Professor, Agricultural and Natural Resource Research Center of Zanajn Province, Zanjan, Iran.
4 Laboratory Technician of Agricultural and Natural Resource Research Center of Zanajn Province, Zanjan, Iran.
Abstract
Peroxidase and Catalyse are two important enzymes involved in reaction of many crop species to salt tolerance. Assessment of activity of these enzymes in the seedlings of barley during salt stress and using quantitative data for mapping of QTLs involved in salt tolerance has been addressed in this study. Parents of four barley mapping populations were exposed to the different concentrations (0 mM, 100mM and 200 mM) of salt (NaCl) and the level of activity of Catalase and Proxidase were quantified in the seedlings stage. There was a high variation in enzymatic activities of parents of OWB population (Dom and Rec) in 200mM of NaCl. In order to map QTLs involved in salt tolerance in OWB, 94 doubled haploid lines were exposed to 0 mM and 200mM of NaCl and the activity of Catalase and Peroxidase were quantified. Mapping of QTLs was performed using MAPQTL5 software. The results showed that the position of QTLs depends on both concentration of salt and on the type of enzyme. For Peroxidase activity there was no QTL mapped despite a high variation between individuals of mapping population while for Catalase in the concentration of 200 mM of NaCl, two QTLS in Chromomes 3(3H) and 4(4H) were mapped. Two other minor QTLs with LOD values slightly lower than the threshold were mapped in chromosomes 5(1H) and 7(5H). The results showed that quantification of the level of activity of Catalase can be used as quantitative data for mapping of QTLs involved in salt tolerance in barley
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