Study of Coumaroyl coenzymeA -3 - hydroxylase Gene expression in different branches of rice roots in response to salinity

Document Type : Research Paper

Authors

1 M.Sc. of Agricultural Biotechnology, Department of Biotechnology, Faculty of Energy Engineering and New Technologies, Shahid Beheshti University, Tehran, Iran.

2 Associate Professor, Department of Biotechnology, Faculty of Energy Engineering and New Technologies, Shahid Beheshti University, Tehran, Iran.

3 Associate Professor, Department of Oil Seed Crops Research, Seed and Plant Improvement Institute. Karaj, Iran.

Abstract

Soil and water salinity is one of the limiting factors for rice cultivation in the worldwide. Among crops, rice is very sensitive to salinity but the sensitivity is higher at the seedling and heading stages. Study of gene expression patterns besides away of cellular proteins function, could be useful to create of resistance plants to stress such as salinity. In this study, expression of the Coumaroyl coenzyme A -3hydroxylase (C3H) gene and physiological processes was surveyed under salinity stress in Oryza sativa cv. IR65192-4B cultivar, by Complete Randomized Design (CRD) in three replicates and qRT-PCR technique. Physiological results showed that, salinity stress reduced root length in 100mM but not significant in 0 and 50 mM NaCl. In addition, reduction of root dry weight was significant under salinity. On the other hand, data analysis of C3H gene expression shown, expression altered in differentages of the rice roots under one level of NaCl, and also, is variable in one age but in different of NaCl concentrations. Weak gene performance in one branch can lead the loss of other branches efficiency. Therefore, this point must be consider to create of salt resistance plant because can play a major role in overall resistance. 

Keywords

Main Subjects

Crop Biotechnology
Volume 3, Issue 2 - Serial Number 5
February 2013
Pages 139-146

Files

Share

How to cite

Statistics