Genome‑wide study of metal tolerance protein (MTP) family in Medicago truncatula , Phaseolus vulgaris and Glycine max .

Pakbaz, Zahra; Ebrahimi, Asa; Rickauer, Martina; Ben, Cecile; Mohammadi, Abdollah

doi:10.30473/cb.2022.60877.1854

Document Type : Research Paper

Authors

¹ Department of Plant Breeding and Biotechnology, Science and Research Branch, Islamic Azad University, Tehran, Iran.

² Laboratoire Ecologie Fonctionnelle et Environnement, Université de Toulouse, CNRS, Toulouse, France.

³ Associate Professor, Department of Plant Breeding, Karaj Branch, Islamic Azad University, Karaj, Iran.

https://doi.org/10.30473/cb.2022.60877.1854

Abstract

Metal tolerance proteins (MTP) are plant membrane divalent cation transporters, which plays an important role during plant growth and development. They involve in minerals uptake and provide resistance for plants in polluted soil by heavy metal. However, information about MTPs proteins in Fabceace family are scarcely known. Therefore, in this study we provided an extensive evaluation of MTP genes in three important members of this family including: Glycine max, Medicago truncatula and Phaseolus vulgaris by providing phylogenetic assessments, chromosomal distributions, gene structures and expression in different tissue. According to the results 14, 12 and 23 MTP genes respectively were found in M. truncatula, P. vulgaris and G. max. 13 duplicated MTP genes in G. max were found meanwhile we did not find any duplication in the MTP genes of M. truncatula and P. vulgaris. All studied MTPs were classified into three major cation diffusion facilitator (CDFs) groups; Mn-CDFs, Zn-CDFs, and Fe/Zn-CDFs. In silico subcellular location results revealed that these proteins have the maximum activity in the vacuole in all three plants, and a small number are located in the cell wall and nucleus. According to gene structure and protein motifs of studied MTPs, they are highly conserved but their expression measurement showed that each one of them have different levels of expression during growth stage. It confirms their importance for plants during growth and development.

Keywords

20.1001.1.22520783.1400.11.35.5.6

Main Subjects

Bioinformatics

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Genome‑wide study of metal tolerance protein (MTP) family in Medicago truncatula , Phaseolus vulgaris and Glycine max .

References

References

Volume 11, Issue 35 - Serial Number 35April 2021Pages 63-77

Volume 11, Issue 35 - Serial Number 35
April 2021
Pages 63-77