Investigating the relationship between morphological and molecular diversity in safflower genotypes

Shahvardi, Mohammad Saleh; Mohsenzadeh, Mohammad; Habibollah Samizadeh Lahiji, Habibollah

doi:10.30473/cb.2023.66492.1896

Investigating the relationship between morphological and molecular diversity in safflower genotypes

Document Type : Research Paper

Authors

¹ M.Sc, Plant Breeding, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran,

² Assistant Professor, Department of Plant Biotechnology, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran.

³ Professor, Department of Plant Biotechnology, Faculty of Agriculture, University of Guilan, Rasht, Iran

10.30473/cb.2023.66492.1896

Abstract

This experiment was conducted in order to assess genetic variation and determination of the best genetic structure and the grouping of safflower genotypes using ISSR and retrotransposon markers and different agricultural traits. In this study, 28 safflower genotypes were evaluated using 7 ISSR markers, 3 retrotransposon markers and 12 combined ISSR and retrotransposon markers, as well as eight different morphological traits with different statistical methods. From 22 primers, 117 polymorphic bands were created. The primers, primer-of UBC-827 and TOS-1 with 13 band maximum number of bands, and primer UBC-811 and UBC-822 in combination with 5-band TOS-1 with the lowest band for ISSR primers and Retrotransposons. The average percent polymorphism obtained in this study for markers ISSR, Retrotransposons and primer combinations was from 38.46 to 88.88 percent ISSR and Retrotransposons is variable and the mean percentage of polymorphic for this is equal to 62. High standards of straw gene diversity, Shannon index, the PIC and the number of effective allele primer UBC-810, UBC-811 and primer combination TOS-2 + HB-12, TOS-1 + HB12 and TOS-1+ UBC822 show performance Primers on the assessment of genetic diversity in this article. The morphological traits of 28 genotypes by UPGMA and Euclidean distance criteria were divided into 4 groups. Grouping results of cluster analysis by linear discriminant analysis using Fisher's focal 82.1 percent for morphological traits were confirmed. Principal component analysis showed that the main vectors of the first and second respectively are 8.22 and 6.84 and the 10 first components validated 63.47 percent of total variance. Mantel test the relationship between molecular and morphological data matrix equivalent 0.214 that show little correlation between the two data. Overall, the results showed that there is a considerable genetic variation in safflower germplasm that can be used to select parents and desirable genotypes in safflower breeding programs.

Keywords

Main Subjects

Molecular Plant Breeding

References

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