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A rapid in vitro micropropagation protocol from nodal segments of Rosa hybrid Cv. City of leads

Document Type : Research Paper

Authors

Abstract

An efficient in vitro propagation protocol of Rosa hybrid Cv. City of leads by axillary shoot proliferation from nodal segments of mature plants was developed. Explants were cultured on two medium formulations, namely Murashige and Skoog (MS) and Van der salm supplemented with various concentrations of N6- benzyladenine (BA) and α-naphthalene acetic acid (NAA). The best explant establishment (100%) and shoot number (3.4 axillary shoots/ explant) was achieved in Van der salm medium supplemented with 2 mg l-1 BA and 0.05 mg l-1 NAA. The following step was to improve the clonal propagation of this cultivar. Multiple shoots were induced from shoot tips cultured on agar based Vander salm medium containing BA (0, 0.5, 1.25, 1.5 and 1.75 mg l-1) alone or in combination with NAA (0, 0.03, 0.06 and 0.09 mg l-1). The highest number (9.6 shoot per explants) of proliferated shoots was obtained with 1.75 mg l-1 BA plus 0.03 mg l-1 NAA. Elongation of the induced shoots was achieved on Van der salm basal medium containing 0, 0.01, 0.05 and 0.1 mg l-1 BA alone or in combination with 0, 0.03, 0.06 and 0.09 mg l-1 NAA. The best treatment for shoot elongation was obtained with 0.01 mg l-1 BA plus 0.09 mg l-1 NAA. Elongated shoots were excised and transferred into rooting medium containing auxins NAA or IBA. The highest percentage (93.0 %) of root induction was obtained on Van der salm medium supplemented with 0.05 mg l-1 NAA.

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References
 
 
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Crop Biotechnology
Volume 2, Issue 2 - Serial Number 2
September 2012
Pages 87-95
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