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Quantitative gene expression of Candidate Genes for Septoria tritici blotch (STB) Resistance in Wheat Infected by Mycosphaerella graminicola

Document Type : Research Paper

Authors

1 Former M.Sc. Student, Payame Noor University, Tehran, Iran

2 Professor, Department of Biology, Faculty of Science, Payame Noor University, Tehran, Iran

3 Assistant Professor, Medical School of Shahroud, Islamic Azad University, Shahroud, Iran

4 Professor, Agricultural Biotechnology Research Institute of Iran, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

Abstract

Septoria tritici blotch (STB), caused by the fungus Mycosphaerella graminicola is one of the most important factors in reducing wheat yield in Iran and the world. However, biotic stress resistance facilitated by candidate genes is one of important selection criteria in wheat breeding programs. In this study, five different families of resistance to the disease were studied. Specific primers were designed using Molecular Beacon software. The differential expression of these genes was investigated at 0, 3, 6, 12 and 24 hours after inoculation.in resistant (Wangshubai) and sensitive cultivars (Seri 82) using quantitative gene expression analysis. The results showed expression level of inhibitors protease Bsi was 24 times higher in Wangshubai than Seri 82 12 hours after inoculation. Further, the expression of protein-related pathogenesis (PR-1) and peroxidase (Per) increased at early hours after infection in Wangshubai. The difference between the expression pattern of the gene linked to resistance mechanisms is the most important step in study of the selection of new cultivars in traditional and modern breeding strategy.

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References
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Crop Biotechnology
Volume 7, Issue 20 - Serial Number 20
March 2018
Pages 75-85
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