اثر ملاتونین بر خصوصیات بیوشیمایی و مقدار بیان ژن‌های مرتبط با فعالیت آنزیم‌های آنتی‌‌اکسیدانت در گندم نان (Triticum aestivum L.) در شرایط تنش کم‌آبی

نوع مقاله : علمی پژوهشی

نویسندگان

1 دانشیار گروه کشاورزی، دانشگاه پیام‌نور، صندوق پستی 3697-19395 تهران، ایران.

2 دانشجوی کارشناسی ارشد، بیوتکنولوژی کشاورزی، دانشگاه پیام‌نور، تهران، ایران.

3 بخش تحقیقات چغندرقند، مرکز تحقیقات کشاورزی و منابع طبیعی همدان، سازمان تحقیقات آموزش و ترویج کشاورزی، همدان، ایران

چکیده

با هدف اثر سطوح مختلف ملاتونین بر خصوصیات بیوشیمیایی و مقدار بیان ژن‌های مرتبط با فعالیت آنزیم‌های آنتی‌‌اکسیدان در گندم نان، آزمایشی به‌صورت اسپلیت پلات در قالب طرح بلوک‌های کامل تصادفی اجرا شد. سطوح آبیاری نرمال ( (FC= 80 درصد)، تنش ملایم (FC= 60 درصد) و تنش شدید (FC= 40 درصد) به کرت‌های اصلی و محلول‌پاشی ملاتونین (صفر ، 50، 100، 150 و 200 μM) به کرت‌های فرعی اختصاص یافتند. نتایج نشان داد با تشدید تنش کم‌‌آبی بر محتوی فلانوئید و مقدار فعالیت آنزیم آسکروبات پراکسیداز افزوده شد و سطح μM‌‌100 ملاتونین بالاترین محتوی فلانوئید، مقدار فعالیت آنزیم آسکروبات پراکسیداز را به خود اختصاص داد. مقایسه میانگین تیمارهای برهمکنش نشان داد بالاترین محتوی پرولین، فنل، سوپر اکسید دیسموتاز و کاتالاز به تیمار محلول‌‌پاشی μM 100 ملاتونین تحت شرایط تنش شدید کم‌آبی اختصاص یافت. همچنین کمترین مقدار مالون‌‌دی‌‌آلدهید برای تیمار محلول‌‌پاشی μM50 ملاتونین تحت شرایط آبیاری نرمال مشاهده شد. بالاترین محتوی کلروفیل a، کلروفیل b، کارتنوئید و عملکرد دانه در تیمار محلول‌‌پاشی 100 میکرومولار ملاتونین و شرایط آبیاری نرمال ثبت شد. در این بررسی حداکثر بیان ژن‌‌های سوپر اکسید دیسموتاز، آسکروبات پراکسیداز، پلی فنل اکسیداز و کاتالاز در تیمار محلول‌‌پاشی سطوح 100 و 150 μM ملاتونین تحت شرایط تنش شدید اختصاص یافت. محلول‌‌پاشی ملاتونین به خصوص سطح μM 100 توانست با بهبود خصوصیات بیوشیمیایی و آنتی اکسیدانی اثر تنش کم‌آبی را بر عملکرد دانه تعدیل نماید.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

The effect of melatonin levels on biochemical properties and the expression of genes related to antioxidant enzymes activity in Triticum aestivum L. under drought stress conditions

نویسندگان [English]

  • Marouf Khalili 1
  • Mohammad Hasso Mohammad 2
  • Hamze Hamze 3
1 Associate Professor, Department of Biotechnology and Plant Breeding, Payame Noor University, Tehran, Iran
2 M.Sc. Student, Department of Crop Science and Biotechnology, Payame Noor, Tehran, Iran.
3 Agricultural and Natural Resources Research Center of Hamedan, Agricultural Research, Education and Extension Organization (AREEO), Iran
چکیده [English]

To study the effect of different levels of melatonin on the biochemical properties and the amount of expression of genes related to the activity of antioxidant enzymes in bread wheat, a split plot experiment was conducted based on a randomized complete Triticum aestivum block design. Irrigation levels (normal (FC = 80%)), mild stress (FC = 60%), and severe stress (FC = 40%)) were allocated to the main plots and melatonin foliar spray (zero, 50, 100, 150, and 200 μM) were assigned to subplots. The results showed that the flavonoid content and ascorbate peroxidase enzyme activity increased with the intensification of dehydration stress. The level of 100 µM melatonin had the highest flavonoid content, ascorbate peroxidase enzyme activity. The mean comparison interaction treatments showed that the highest content of proline, phenol, superoxide dismutase and catalase was assigned to the 100 µM melatonin foliar spray under conditions of extreme stress of dehydration. Also, the lowest amount of malondialdehyde was observed for the 50 μM melatonin foliar treatment under normal irrigation conditions. The highest content of chlorophyll a, chlorophyll b, carotenoid and grain yield were recorded in the treatment of foliar spray of 100 μM melatonin and normal irrigation conditions. In this investigation, the maximum expression of superoxide dismutase, ascorbate peroxidase, polyphenol oxidase and catalase genes were determined in the foliar spray of 100 and 150 μM melatonin levels under conditions of extreme stress. . The foliar spray of melatonin, especially at the level of 100 μM, could moderate the effect dehydration stress on grain yield by improving biochemical and antioxidant properties.

کلیدواژه‌ها [English]

  • antioxidant
  • gene expression
  • melatonin
  • water deficit
  • wheat
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