ردیابی ژن(های) طول دانه برنج در جمعیت نسل دوم با استفاده از نشانگرهای مورفولوژیکی و ‏مولکولی

نوع مقاله : علمی پژوهشی

نویسندگان

دانشگاه علوم کشاورزی و منابع طبیعی ساری

چکیده

طول دانه یکی از خصوصیات بسیار مهم در اصلاح برنج است که بر عملکرد و ویژگی‌های کیفی دانه تأثیر می‌گذارد. در این مطالعه تنوع ژنتیکی صفات طول و عرض و وزن هزار دانه در جمعیت‌ F2 حاصل از تلاقی لاین L44 (والد مادری) و ژنوتیپ IR-229R (والد پدری) مورد ارزیابی قرار گرفت. همچنین از نشانگرهای مولکولی هم‌بسته با طول دانه برای شناسایی ژنوتیپ‎هایی با طول دانه بلندتر در جمعیت F2 استفاده شد. نتایج ارزیابی صفات مورفولوژی نشان داد که میانگین طول دانه برنج در جمعیت نسل دوم L44 × IR-229R برابر با 16/11 میلی‌متر می‌باشد که به میانگین طول دانه در ژنوتیپ مادریL44 (11 میلی‌متر) نزدیک است. همچنین تعداد 10 ژنوتیپ طول دانه بلندتر از والد پدری نشان دادند که از بین این ژنوتیپ‌ها، تعداد 6 ژنوتیپ (شماره‌های 8، 10، 76، 82، 91 و 96) عرض دانه و وزن هزار دانه بیشتری از میانگین جمعیت داشتند. در ارزیابی مولکولی مشخص شد که آغازگرهای RM488 و RM234 (به ترتیب هم‌بسته با طول دانه برنج روی کروموزم 1 و 7) بین ژنوتیپ‌های والدینی چندشکلی نشان دادند. در بررسی صفت طول دانه با نشانگرهای RM488 و RM234، ژنوتیپ‌های 76، 82، 91 و 101 با طول دانه به ترتیب 96/12، 66/12، 79/12 و 53/12 میلی‌متر بعنوان ژنوتیپ‌های دانه بلند شناسایی شدند.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Detection of rice grain length genes in the second-generation population using morphological and molecular markers

نویسندگان [English]

  • nadali bagheri
  • zeinab Masoudi Jozchal
Sari Agricultural Sciences and Natural Resources University
چکیده [English]

Grain length is one of the most important characteristics in rice breeding, which affects the yield and quality of the grain. In this study, the genetic diversity of grain length and width and 1000 seed weight were evaluated in the F2 population resulting from the crossing of L44 line (maternal parent) and IR-229R genotype (paternal parent). Also, molecular markers correlated with grain length were used to identify genotypes with longer grain length in the F2 population. The results of the evaluation of morphological traits showed that the average length of rice grain in the second generation L44 × IR-229R population is 11.16 mm, which is close to the average grain length in the mother genotype L44 (11 mm). Also, 10 genotypes showed longer seed length than the paternal parent, and among these genotypes, 6 genotypes (numbers 8, 10, 76, 82, 91 and 96) had greater seed width and 1000 seed weight than the population average. In the molecular evaluation, it was found that primers RM488 and RM234 (correlated with rice grain length on chromosome 1 and 7, respectively) showed polymorphism between parent’s genotypes. In examining the grain length trait with markers RM488 and RM234, genotypes 76, 82, 91 and 101 with grain length of 12.96, 12.66, 12.79 and 12.53 mm respectively were identified as long seed genotypes.

کلیدواژه‌ها [English]

  • Aggressive separation
  • F2 population
  • Grain shape
  • Polymorphism
  • Rice
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