بررسی خصوصیات رشدی و فیتوشیمیایی در کشت درون‌شیشه‌ای Nitraria schoberi ‎ تحت تنش شوری

نوع مقاله : علمی پژوهشی

نویسندگان

1 گروه کشاورزی، دانشگاه پیام نور، تهران، ایران

2 مرکز تحقیقات گیاهان دارویی، پژوهشکده گیاهان دارویی، جهاد دانشگاهی، کرج، ایران.

3 گروه بیونکنولوژی کشاورزی، دانشگاه پیام نور، تهران، ایران

4 مرکز تحقیقات گیاهان دارویی، پژوهشکده گیاهان دارویی، جهاد دانشگاهی، کرج، ایران

5 دانشجوی دکتری، بانک گیاهی، مرکز ملی ذخایر ژنتیکی و زیستی ایران، جهاد دانشگاهی، کرج، ایران

چکیده

به منظور بررسی اثر تنش شوری بر خصوصیات رشدی و فیتوشیمیایی در کشت درون شیشه‌ای گیاه قره‌داغ (Nitraria schoberi) آزمایشی در قالب طرح فاکتوریل با پایه کاملا تصادفی انجام شد. تیمارها شامل غلظت‌های مختلف کلرید‌سدیم (0، 50 و 100 میلی‌مولار) و سولفات‌سدیم (0، 10 و 20میلی‌مولار) بود. ابتدا بذر‌ها در شرایط درون‌شیشه‌ای جوانه دار شدند و سپس قسمت‌های برگ‌های کوتیلدونی به همراه هیپوکوتیل بذرهای جوانه‌زده به عنوان ریزنمونه در محیط کشت MS به همراه 2 میلی گرم در لیتر BAP ، 5/0 میلی گرم در لیتر IBA و تیمارهای ذکر شده قرار گرفتند. ریزنمونه‌های آماده شده در اتاق رشد درشرایط 16 ساعت روشنایی و 8 ساعت تاریکی دمای 2±25 قرار داده شدند. پس از 4 هفته طول ساقه، تعداد برگ و میزان فنل و فلاونویید کل اندازه گیری شد. نتایج تجزیه واریانس نشان داد که سطوح مختلف نمک بر شاخص‌های رشدی گیاهچه‌ها چون طول ساقه و تعداد برگ‌ها و همینطور بر میزان فنل و فلاونوئید کل موجود در بافت برگ‌ها تاثیر معنی‌داری (در سطح 99 درصد) دارد. بر اساس مقایسات میانگین بیشترین طول ساقه ‌(3/4 سانتی متر) و تعداد برگ (5/5) مربوط به محیط کشت فاقد نمک و بیشترین میزان فنل کل (37/35 میلی گرم در گرم) و فلاونوئید کل (6/24 میلی گرم در گرم) مربوط به محیط کشت حاوی 50 میلی‌مولار کلرید‌سدیم به همراه 10 میلی‌مولار سولفات‌سدیم بوده است.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Investigating the growth and phytochemical characteristics in the in vitro culture of Nitraria schoberi under salt stress

نویسندگان [English]

  • Hamideh Khalaj 1
  • nassim zarinpanjeh 2
  • Mojtaba Askarizadeh 3
  • Hadi Kalantari Khalilabad 4
  • Javad Shahghaghi 5
1 ., Department of Agriculture, Pyamen Noor University, Tehran, Iran
2 Medicinal Plants Research Center, ‎Institute of medicinal plants, ACECR, ‎Karaj, Iran.
3 Department of Agriculture, Pyamen Noor University, Tehran, Iran
4 , Medicinal plants Research Center, Institute of medicinal plants, ACECR, Karaj, Iran
5 PhD Student, Plant Bank, Iranian Biological Resource Center (IBRC), ACECR, Karaj, Iran
چکیده [English]

To investigate the effect of salt stress on the growth and phytochemical characteristics in the in vitro cultivation of Nitraria schoberi, an experiment was conducted in the form of a factorial design with a completely random basis. The treatments included different concentrations of sodium chloride (0, 50 and 100 mM) and sodium sulfate (0, 10 and 20 mM). First, the seeds were germinated in vitro and then the cotyledon leaves with the hypocotyl of germinated seeds were placed as explants in MS cultivation environment with 2 mg/L BAP, 0.5 mg/L IBA, and the mentioned treatments. The explants were placed in the growth room under the conditions of 16 hours of light and 8 hours of darkness at a temperature of 25±2. After 4 weeks, stem length, number of leaves, and total phenol and flavonoids were measured. The results of variance analysis showed that different levels of salt have a significant effect (at the 99% level) on the growth indices of seedlings such as the length of the stem and the number of leaves, as well as on the total amount of phenol and flavonoid in the tissue of leaves. Based on comparisons, the maximum length of the stem (4.3 cm) and the number of leaves (5.5) related to the cultivation environment without salt and the highest amount of total phenol (35.37 mg/g) and flavonoid (24.6 mg/g) g/g) related to the cultivation environment containing 50 mM sodium chloride with 10 mM sodium sulfate.

کلیدواژه‌ها [English]

  • Sodium sulfate
  • total flavonoid
  • total phenol
  • sodium chloride
  • Qara-e-Dagh (Nitraria schoberi)

مراجع

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